Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells

Giancarlo Marra, Christina L. Chang, Luigi A. Laghi, Dharam P. Chauhan, Dennis Young, C. Richard Boland

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The hMSH2 protein plays an important role in the DNA mismatch repair system. Since this system is involved in the correction of errors that occur during DNA replication, we studied the expression of hMSH2 protein in resting and DNA-replicating cells, as well as through the cell cycle in cell types with different growth characteristics. Using Western blot analysis, we showed that hMSH2 protein was detectable in resting peripheral blood lymphocytes and thymocytes. However, when these cells mere induced to proliferate, the protein level increased at least 12-fold. In cell-cycle dependent expression studies we chose two DNA mismatch repair proficient cell lines (HEL and HeLa-S3), and flow cytometry was used to monitor cell-cycle progression. At every phase in the cell cycle, the steady-state level of hMSH2 was higher than in resting lymphocytes or thymocytes, and only minor variations of expression level were observed through the cell cycle. In particular, a two to fourfold decrease in hMSH2 expression occurred at G1 in HEL and at early S phase in HeLa-S3, but higher expression levels resumed during the replicative and postreplicative phases of the cell cycle. Interestingly, hMSH2 protein expression decreased fourfold when HEL cells were induced to differentiate along the megakaryocyte lineage, when continuous DNA replication occurs without mitosis. These results suggest that a basal level of hMSH2 protein expression is necessary for resting and differentiated cells, and that increased hMSH2 protein expression is required when DNA replication is activated and followed by mitosis.

Original languageEnglish
Pages (from-to)2189-2196
Number of pages8
JournalOncogene
Volume13
Issue number10
Publication statusPublished - 1996

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MutS Homolog 2 Protein
Cell Cycle
DNA Replication
DNA Mismatch Repair
Thymocytes
Mitosis
Lymphocytes
Megakaryocytes
S Phase

Keywords

  • Cell cycle
  • DNA mismatch repair
  • hMSH2
  • Protein expression

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Marra, G., Chang, C. L., Laghi, L. A., Chauhan, D. P., Young, D., & Boland, C. R. (1996). Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells. Oncogene, 13(10), 2189-2196.

Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells. / Marra, Giancarlo; Chang, Christina L.; Laghi, Luigi A.; Chauhan, Dharam P.; Young, Dennis; Boland, C. Richard.

In: Oncogene, Vol. 13, No. 10, 1996, p. 2189-2196.

Research output: Contribution to journalArticle

Marra, G, Chang, CL, Laghi, LA, Chauhan, DP, Young, D & Boland, CR 1996, 'Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells', Oncogene, vol. 13, no. 10, pp. 2189-2196.
Marra G, Chang CL, Laghi LA, Chauhan DP, Young D, Boland CR. Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells. Oncogene. 1996;13(10):2189-2196.
Marra, Giancarlo ; Chang, Christina L. ; Laghi, Luigi A. ; Chauhan, Dharam P. ; Young, Dennis ; Boland, C. Richard. / Expression of human MutS homolog 2 (hMSH2) protein in resting and proliferating cells. In: Oncogene. 1996 ; Vol. 13, No. 10. pp. 2189-2196.
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