Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma

Luigi P. Ruco, Petronella A J M De Laat, Cristian Matteucci, Sergio Bernasconi, Francesca Maria Sciacca, Theo H. Van Der Kwast, Henk C. Hoogsteden, Stefania Uccini, Alberto Mantovani, Marjan A. Versnel

Research output: Contribution to journalArticle

Abstract

Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are cytokine-inducible adhesion molecules which recognize ligands that are highly expressed on leukocytes. Expression of ICAM-1 and VCAM- 1 was investigated in tissue sections of 16 cases of malignant mesothelioma (seven epithelial, eight biphasic, and one sarcomatoid) using immunohistochemistry. Neoplastic cells were diffusely and intensely stained for ICAM-1 in all cases. VCAM-1 was detected in 14 of 16 cases. The percentage of VCAM-1-positive tumour cells was more than 50 per cent in eight cases and the staining was observed mainly in epithelial-like cells. VCAM-1 was rarely expressed in other malignant tumours of epithelial origin, being present in only 1 of 58 cases of carcinoma originating from different anatomical sites. At the cellular level, ICAM-1 and VCAM-1 appeared co-distributed, the staining for both being cytoplasmic with a membrane reinforcement. The regulation of VCAM-1 expression by neoplastic mesothelial cells was investigated in vitro using 14 mesothelioma cell lines. ICAM-1 was expressed by cultured cells of all mesothelioma cell lines, even in the absence of cytokines. VCAM-1 was detected in 10-50 per cent of the cells in three non-stimulated mesothelioma cell lines (mero-95, mero-96, and mero-134), and was absent or poorly expressed in the remaining 11. Exposure of a negative cell line (mero-48a) to an optimal concentration of tumour necrosis factor alpha (TNFα) or interleukin-13 (IL- 13) for 6-18 h resulted in the induction of VCAM-1 mRNA synthesis and in VCAM- 1 expression at the membrane level in 60-70 per cent of the cells. These findings are consistent with the possibility that TNFα, IL-13, or other activating signals are released in the turnout micro-environment and regulate the expression of VCAM-1 in malignant mesothelioma cells.

Original languageEnglish
Pages (from-to)266-271
Number of pages6
JournalJournal of Pathology
Volume179
Issue number3
DOIs
Publication statusPublished - 1996

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Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Mesothelioma
Cell Line
Interleukin-13
Malignant Mesothelioma
Tumor Necrosis Factor-alpha
Staining and Labeling
Cytokines
Carcinoma
Membranes
Cultured Cells
Interleukin-6
Leukocytes
Epithelial Cells
Immunohistochemistry
Ligands

Keywords

  • intercellular adhesion molecule-1 (ICAM-1)
  • malignant mesothelioma
  • regulation of expression
  • vascular cell adhesion molecule-1 (VCAM-1)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ruco, L. P., De Laat, P. A. J. M., Matteucci, C., Bernasconi, S., Sciacca, F. M., Van Der Kwast, T. H., ... Versnel, M. A. (1996). Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma. Journal of Pathology, 179(3), 266-271. https://doi.org/10.1002/(SICI)1096-9896(199607)179:3<266::AID-PATH592>3.0.CO;2-Y

Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma. / Ruco, Luigi P.; De Laat, Petronella A J M; Matteucci, Cristian; Bernasconi, Sergio; Sciacca, Francesca Maria; Van Der Kwast, Theo H.; Hoogsteden, Henk C.; Uccini, Stefania; Mantovani, Alberto; Versnel, Marjan A.

In: Journal of Pathology, Vol. 179, No. 3, 1996, p. 266-271.

Research output: Contribution to journalArticle

Ruco, LP, De Laat, PAJM, Matteucci, C, Bernasconi, S, Sciacca, FM, Van Der Kwast, TH, Hoogsteden, HC, Uccini, S, Mantovani, A & Versnel, MA 1996, 'Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma', Journal of Pathology, vol. 179, no. 3, pp. 266-271. https://doi.org/10.1002/(SICI)1096-9896(199607)179:3<266::AID-PATH592>3.0.CO;2-Y
Ruco, Luigi P. ; De Laat, Petronella A J M ; Matteucci, Cristian ; Bernasconi, Sergio ; Sciacca, Francesca Maria ; Van Der Kwast, Theo H. ; Hoogsteden, Henk C. ; Uccini, Stefania ; Mantovani, Alberto ; Versnel, Marjan A. / Expression of ICAM-1 and VCAM-1 in human malignant mesothelioma. In: Journal of Pathology. 1996 ; Vol. 179, No. 3. pp. 266-271.
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