Expression of IL-2 receptors in human melanoma cells

D. Rimoldi, S. Salvi, F. Hartmann, M. Schreyer, S. Blum, L. Zografos, S. Plaisance, B. Azzarone, S. Carrel

Research output: Contribution to journalArticlepeer-review

Abstract

Melanoma cells can secrete several cytokines and express various cell surface molecules such as the intercellular adhesion molecule ICAM-1, class II histocompatibility antigens, and the CALLA antigen, typically found in cells of the immune system. We have investigated the possible expression of interleukin-2 (IL-2) receptors in melanoma using monoclonal antibodies specific for the p55/α chain (TAC antigen) and the p75/β subunit. Flow cytometric analysis of cultured melanoma cells showed the presence of low levels of the TAC antigen and of the β chain on the surface of several cell lines. Similar results were obtained in vivo by immunohistochemistry on cryosections prepared from cutaneous and ocular melanoma explants. Positive staining was observed for the a chain of the IL-2 receptor in a high percentage of tumour cells. The β chain could also be detected, although in a limited number of specimens. Analysis of RNA from melanoma cell lines by Northern blot showed the presence of typical 4 Kb transcripts for the p75 subunit, while low-abundance message for the p55 chain could be detected using combined reverse transcription/polymerase chain reaction analysis. Together these results suggest that melanoma cells may express high affinity receptors for IL-2.

Original languageEnglish
Pages (from-to)555-564
Number of pages10
JournalAnticancer Research
Volume13
Issue number3
Publication statusPublished - 1993

Keywords

  • Human melanoma
  • IL-2
  • IL-2 receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint

Dive into the research topics of 'Expression of IL-2 receptors in human melanoma cells'. Together they form a unique fingerprint.

Cite this