Expression of interleukin 15 (IL-15) in human rhabdomyosarcoma, osteosarcoma and Ewing's sarcoma

Pier Luigi Lollini, Gabriella Palmieri, Carla De Giovanni, Lorena Landuzzi, Giordano Nicoletti, Ilaria Rossi, Cristiana Griffoni, Flavia Frabetti, Katia Scotlandi, Stefania Benini, Nicola Baldini, Angela Santoni, Patrizia Nanni

Research output: Contribution to journalArticle

Abstract

Interleukin 15 (IL-15) is a recently discovered cytokine that stimulates lymphocyte proliferation and migration via a trimeric receptor sharing the β and γ signal transducing chains with the IL-2 receptor. IL-15 is typically produced by normal cells that do not release IL-2, but little information is currently available on human tumors. To assess whether human musculo-skeletal sarcomas produce IL-15, we analyzed surgical specimens and cell lines obtained from rhabdomyosarcoma, osteosarcoma and Ewing's sarcoma. IL-15 mRNA was present in 9/9 surgical specimens (3 Ewing's sarcomas, 5 osteosarcomas and 1 rhabdomyosarcoma). The analysis of a panel of cell lines (7 derived from Ewing's sarcoma, 12 from osteosarcoma and 5 from rhabdomyosarcoma) showed that all rhabdomyosarcoma and osteosarcoma cell lines expressed IL-15 mRNA at levels ranging from low to high, while Ewing's sarcoma cells contained little or no IL-15 message, ELISA assays showed IL-15 release in a subset of rhabdomyosarcomas and osteosarcomas, but not in Ewing's sarcoma. The highest production of IL-15, in the picogram/ml range, was found in rhabdomyosarcoma cell lines RH30 and RD.

Original languageEnglish
Pages (from-to)732-736
Number of pages5
JournalInternational Journal of Cancer
Volume71
Issue number5
DOIs
Publication statusPublished - 1997

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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