Expression of kidney and liver bilitranslocase in response to acute biliary obstruction

Anabel Brandoni, Gisela Di Giusto, Raffaella Franca, Sabina Passamonti, Adriana M. Torres

Research output: Contribution to journalArticle

Abstract

Background/Aim: It has been recently demonstrated that acute obstructive jaundice is associated with modifications in the renal expression and function of organic anion transporters such as Oat1, Oat3, Oatp1 and Mrp2. This study examined the expression and function of bilitranslocase in liver and kidney from rats with bile duct ligation (BDL). Methods: Bilitranslocase expression was evaluated in renal homogenates (H), renal basolateral plasma membranes (KBLM) and liver plasma membranes (LPM) by immunoblotting. Bilitranslocase function was studied by measuring the kinetic parameters of electrogenic bromosulfophthalein (BSP) uptake in KBLM and LPM by a spectrophotometric technique. Results: An increased abundance of bilitranslocase in KBLM without modifications in renal H and in LPM from BDL rats was observed compared with Sham rats. BDL rats showed a higher V max for BSP uptake in KBLM. No differences between groups were observed for Michaelis-Menten parameters in LPM. Conclusion: The higher renal expression and function of bilitranslocase in renal basolateral membranes from rats with obstructive cholestasis might also contribute to the dramatic increase in BSP renal excretion observed in this experimental model. This would be another compensation mechanism to overcome the hepatic dysfunction in the elimination of organic anions.

Original languageEnglish
JournalNephron - Physiology
Volume114
Issue number4
DOIs
Publication statusPublished - Apr 2010

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Keywords

  • Acute jaundice
  • Bile duct obstruction
  • Bilitranslocase
  • Cholestasis
  • Organic anion transporters

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)

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