Expression of KIF3C kinesin during neural development and in vitro neuronal differentiation

Francesca Navone, G. Giacomo Consalez, Milena Sardella, Elisabetta Caspani, Ombretta Pozzoli, Carolina Frassoni, Elena Morlacchi, Roberto Sitia, Teresa Sprocati, Andrea Cabibbo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

KIF3A, KIF3B and KIF3C are kinesin-related motor subunits of the KIF3 family that associate to form the kinesin-II motor complex in which KIF3C and KIF3B are alternative partners of KIF3A. We have analysed the expression of Kif3 mRNAs during prenatal murine development. Kif3c transcripts are detectable from embryonic day 12.5 and persist throughout development both in the CNS and in some peripheral ganglia. Comparison of the expression patterns of the Kif3 genes revealed that Kif3c and Kif3a mRNAs colocalize in the CNS, while only Kif3a is also present outside the CNS. In contrast, Kif3b is detectable in several non-neural tissues. We have also performed immunocytochemical analyses of the developing rat brain and have found the presence of the KIF3C protein in selected brain regions and in several fibre systems. Using neuroblastoma cells as an in vitro model for neuronal differentiation, we found that retinoic acid stimulated the expression of the three Kif3 and the kinesin-associated protein genes, although with different time courses. The selective expression of Kif3c in the nervous system during embryonic development and its up-regulation during neuroblastoma differentiation suggest a role for this motor during maturation of neuronal cells.

Original languageEnglish
Pages (from-to)741-753
Number of pages13
JournalJournal of Neurochemistry
Volume77
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Kinesin
Neuroblastoma
Messenger RNA
Brain
Tretinoin
Ganglia
Nervous System
Embryonic Development
Proteins
Up-Regulation
Neurology
Rats
Genes
Tissue
Fibers
In Vitro Techniques
kinesin-II

Keywords

  • Brain development
  • Kinesin II
  • Microtubule-based motors
  • Mouse development
  • Neuroblastoma cells
  • Neuronal differentiation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Expression of KIF3C kinesin during neural development and in vitro neuronal differentiation. / Navone, Francesca; Consalez, G. Giacomo; Sardella, Milena; Caspani, Elisabetta; Pozzoli, Ombretta; Frassoni, Carolina; Morlacchi, Elena; Sitia, Roberto; Sprocati, Teresa; Cabibbo, Andrea.

In: Journal of Neurochemistry, Vol. 77, No. 3, 2001, p. 741-753.

Research output: Contribution to journalArticle

Navone, Francesca ; Consalez, G. Giacomo ; Sardella, Milena ; Caspani, Elisabetta ; Pozzoli, Ombretta ; Frassoni, Carolina ; Morlacchi, Elena ; Sitia, Roberto ; Sprocati, Teresa ; Cabibbo, Andrea. / Expression of KIF3C kinesin during neural development and in vitro neuronal differentiation. In: Journal of Neurochemistry. 2001 ; Vol. 77, No. 3. pp. 741-753.
@article{5b828ec03bcd4684a3518f7af2660425,
title = "Expression of KIF3C kinesin during neural development and in vitro neuronal differentiation",
abstract = "KIF3A, KIF3B and KIF3C are kinesin-related motor subunits of the KIF3 family that associate to form the kinesin-II motor complex in which KIF3C and KIF3B are alternative partners of KIF3A. We have analysed the expression of Kif3 mRNAs during prenatal murine development. Kif3c transcripts are detectable from embryonic day 12.5 and persist throughout development both in the CNS and in some peripheral ganglia. Comparison of the expression patterns of the Kif3 genes revealed that Kif3c and Kif3a mRNAs colocalize in the CNS, while only Kif3a is also present outside the CNS. In contrast, Kif3b is detectable in several non-neural tissues. We have also performed immunocytochemical analyses of the developing rat brain and have found the presence of the KIF3C protein in selected brain regions and in several fibre systems. Using neuroblastoma cells as an in vitro model for neuronal differentiation, we found that retinoic acid stimulated the expression of the three Kif3 and the kinesin-associated protein genes, although with different time courses. The selective expression of Kif3c in the nervous system during embryonic development and its up-regulation during neuroblastoma differentiation suggest a role for this motor during maturation of neuronal cells.",
keywords = "Brain development, Kinesin II, Microtubule-based motors, Mouse development, Neuroblastoma cells, Neuronal differentiation",
author = "Francesca Navone and Consalez, {G. Giacomo} and Milena Sardella and Elisabetta Caspani and Ombretta Pozzoli and Carolina Frassoni and Elena Morlacchi and Roberto Sitia and Teresa Sprocati and Andrea Cabibbo",
year = "2001",
doi = "10.1046/j.1471-4159.2001.00277.x",
language = "English",
volume = "77",
pages = "741--753",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Expression of KIF3C kinesin during neural development and in vitro neuronal differentiation

AU - Navone, Francesca

AU - Consalez, G. Giacomo

AU - Sardella, Milena

AU - Caspani, Elisabetta

AU - Pozzoli, Ombretta

AU - Frassoni, Carolina

AU - Morlacchi, Elena

AU - Sitia, Roberto

AU - Sprocati, Teresa

AU - Cabibbo, Andrea

PY - 2001

Y1 - 2001

N2 - KIF3A, KIF3B and KIF3C are kinesin-related motor subunits of the KIF3 family that associate to form the kinesin-II motor complex in which KIF3C and KIF3B are alternative partners of KIF3A. We have analysed the expression of Kif3 mRNAs during prenatal murine development. Kif3c transcripts are detectable from embryonic day 12.5 and persist throughout development both in the CNS and in some peripheral ganglia. Comparison of the expression patterns of the Kif3 genes revealed that Kif3c and Kif3a mRNAs colocalize in the CNS, while only Kif3a is also present outside the CNS. In contrast, Kif3b is detectable in several non-neural tissues. We have also performed immunocytochemical analyses of the developing rat brain and have found the presence of the KIF3C protein in selected brain regions and in several fibre systems. Using neuroblastoma cells as an in vitro model for neuronal differentiation, we found that retinoic acid stimulated the expression of the three Kif3 and the kinesin-associated protein genes, although with different time courses. The selective expression of Kif3c in the nervous system during embryonic development and its up-regulation during neuroblastoma differentiation suggest a role for this motor during maturation of neuronal cells.

AB - KIF3A, KIF3B and KIF3C are kinesin-related motor subunits of the KIF3 family that associate to form the kinesin-II motor complex in which KIF3C and KIF3B are alternative partners of KIF3A. We have analysed the expression of Kif3 mRNAs during prenatal murine development. Kif3c transcripts are detectable from embryonic day 12.5 and persist throughout development both in the CNS and in some peripheral ganglia. Comparison of the expression patterns of the Kif3 genes revealed that Kif3c and Kif3a mRNAs colocalize in the CNS, while only Kif3a is also present outside the CNS. In contrast, Kif3b is detectable in several non-neural tissues. We have also performed immunocytochemical analyses of the developing rat brain and have found the presence of the KIF3C protein in selected brain regions and in several fibre systems. Using neuroblastoma cells as an in vitro model for neuronal differentiation, we found that retinoic acid stimulated the expression of the three Kif3 and the kinesin-associated protein genes, although with different time courses. The selective expression of Kif3c in the nervous system during embryonic development and its up-regulation during neuroblastoma differentiation suggest a role for this motor during maturation of neuronal cells.

KW - Brain development

KW - Kinesin II

KW - Microtubule-based motors

KW - Mouse development

KW - Neuroblastoma cells

KW - Neuronal differentiation

UR - http://www.scopus.com/inward/record.url?scp=0034999277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034999277&partnerID=8YFLogxK

U2 - 10.1046/j.1471-4159.2001.00277.x

DO - 10.1046/j.1471-4159.2001.00277.x

M3 - Article

C2 - 11331403

AN - SCOPUS:0034999277

VL - 77

SP - 741

EP - 753

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 3

ER -