Expression of LINE-1 retroposons is essential for murine preimplantation development

Rosanna Beraldi, Carmine Pittoggi, Ilaria Sciamanna, Elisabetta Mattei, Corrado Spadafora

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

In higher eukaryotes, reverse transcriptase (RT) activities are encoded by a variety of endogenous retroviruses and retrotransposable elements. We previously found that mouse preimplantation embryos are endowed with an endogenous RT activity. Inhibition of that activity by the non nucleosidic inhibitor nevirapine or by microinjection of anti-RT antibody caused early embryonic developmental arrest. Those experiments indicated that RT is required for early development, but did not identify the responsible coding elements. We now show that microinjection of morpholino-modified antisense oligonucleotides targeting the 5′ end region of active LINE-1 retrotransposons in murine zygotes irreversibly arrests preimplantation development at the two- and four-cell stages; the overall level of functional RT is concomitantly downregulated in arrested embryos. Furthermore, we show that the induction of embryo developmental arrest is associated with a substantial reprogramming of gene expression. Together, these results support the conclusion that expression of LINE-1 retrotransposons is required for early embryo preimplantation development.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalMolecular Reproduction and Development
Volume73
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Retroelements
RNA-Directed DNA Polymerase
Microinjections
Embryonic Structures
Endogenous Retroviruses
Nevirapine
Morpholinos
Antisense Oligonucleotides
Zygote
Blastocyst
Eukaryota
Embryonic Development
Down-Regulation
Gene Expression
Antibodies

Keywords

  • Developmental biology
  • Early embryogenesis
  • Gene regulation
  • LINE-1/L1 retrotransposon
  • Reverse transcriptase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Expression of LINE-1 retroposons is essential for murine preimplantation development. / Beraldi, Rosanna; Pittoggi, Carmine; Sciamanna, Ilaria; Mattei, Elisabetta; Spadafora, Corrado.

In: Molecular Reproduction and Development, Vol. 73, No. 3, 03.2006, p. 279-287.

Research output: Contribution to journalArticle

Beraldi, R, Pittoggi, C, Sciamanna, I, Mattei, E & Spadafora, C 2006, 'Expression of LINE-1 retroposons is essential for murine preimplantation development', Molecular Reproduction and Development, vol. 73, no. 3, pp. 279-287. https://doi.org/10.1002/mrd.20423
Beraldi, Rosanna ; Pittoggi, Carmine ; Sciamanna, Ilaria ; Mattei, Elisabetta ; Spadafora, Corrado. / Expression of LINE-1 retroposons is essential for murine preimplantation development. In: Molecular Reproduction and Development. 2006 ; Vol. 73, No. 3. pp. 279-287.
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