Expression of LINE-1 retroposons is essential for murine preimplantation development

Rosanna Beraldi, Carmine Pittoggi, Ilaria Sciamanna, Elisabetta Mattei, Corrado Spadafora

Research output: Contribution to journalArticle

Abstract

In higher eukaryotes, reverse transcriptase (RT) activities are encoded by a variety of endogenous retroviruses and retrotransposable elements. We previously found that mouse preimplantation embryos are endowed with an endogenous RT activity. Inhibition of that activity by the non nucleosidic inhibitor nevirapine or by microinjection of anti-RT antibody caused early embryonic developmental arrest. Those experiments indicated that RT is required for early development, but did not identify the responsible coding elements. We now show that microinjection of morpholino-modified antisense oligonucleotides targeting the 5′ end region of active LINE-1 retrotransposons in murine zygotes irreversibly arrests preimplantation development at the two- and four-cell stages; the overall level of functional RT is concomitantly downregulated in arrested embryos. Furthermore, we show that the induction of embryo developmental arrest is associated with a substantial reprogramming of gene expression. Together, these results support the conclusion that expression of LINE-1 retrotransposons is required for early embryo preimplantation development.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalMolecular Reproduction and Development
Volume73
Issue number3
DOIs
Publication statusPublished - Mar 2006

Keywords

  • Developmental biology
  • Early embryogenesis
  • Gene regulation
  • LINE-1/L1 retrotransposon
  • Reverse transcriptase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of LINE-1 retroposons is essential for murine preimplantation development'. Together they form a unique fingerprint.

  • Cite this