Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors

L. Alberti, L. Gilardini, A. Zulian, G. Micheletto, G. Peri, A. Doni, A. Mantovani, C. Invitti

Research output: Contribution to journalArticle

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Abstract

Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4 ± 8.15 yr) and 10 normal weight subjects (43.7 ± 11.07 yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor α (TNFα) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNFα and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNFα and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only (p = 0.05). CD68 and PTX3 expressions were correlated with each other in SAT but not in VAT. After adjustment for age and sex, VAT-PTX3 expression and release were correlated with VAT-TNFα expression (p <0.01 for both) and with LDL/HDL ratio (p <0.01 and p <0.001). VAT-PTX3 expression was also correlated with BMI, triglycerides, CRP, fibrinogen and adiponectin (p <0.05 for all). In the multivariate analysis with VAT-PTX3 RNA levels as dependent variable, LDL/HDL ratio and fibrinogen remained independently associated with VAT-PTX3 expression (p <0.01 for both). These associations were not seen within SAT. Conclusions: Human adipose tissue expresses and releases PTX3 likely under TNFα control. VAT production of PTX3 seems to contribute to the mechanisms underlying the development of atherosclerosis.

Original languageEnglish
Pages (from-to)455-460
Number of pages6
JournalAtherosclerosis
Volume202
Issue number2
DOIs
Publication statusPublished - Feb 2009

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Adipose Tissue
Intra-Abdominal Fat
Adiponectin
Tumor Necrosis Factor-alpha
Subcutaneous Fat
Fibrinogen
PTX3 protein
C-Reactive Protein
Weights and Measures
Acute-Phase Proteins
LDL Cholesterol
HDL Cholesterol
Real-Time Polymerase Chain Reaction
Atherosclerosis
Triglycerides
Multivariate Analysis
Fats
Macrophages
RNA
Insulin

Keywords

  • Adiponectin
  • Adipose tissue
  • Fibrinogen
  • HDL
  • PTX3

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors. / Alberti, L.; Gilardini, L.; Zulian, A.; Micheletto, G.; Peri, G.; Doni, A.; Mantovani, A.; Invitti, C.

In: Atherosclerosis, Vol. 202, No. 2, 02.2009, p. 455-460.

Research output: Contribution to journalArticle

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abstract = "Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4 ± 8.15 yr) and 10 normal weight subjects (43.7 ± 11.07 yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor α (TNFα) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNFα and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNFα and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only (p = 0.05). CD68 and PTX3 expressions were correlated with each other in SAT but not in VAT. After adjustment for age and sex, VAT-PTX3 expression and release were correlated with VAT-TNFα expression (p <0.01 for both) and with LDL/HDL ratio (p <0.01 and p <0.001). VAT-PTX3 expression was also correlated with BMI, triglycerides, CRP, fibrinogen and adiponectin (p <0.05 for all). In the multivariate analysis with VAT-PTX3 RNA levels as dependent variable, LDL/HDL ratio and fibrinogen remained independently associated with VAT-PTX3 expression (p <0.01 for both). These associations were not seen within SAT. Conclusions: Human adipose tissue expresses and releases PTX3 likely under TNFα control. VAT production of PTX3 seems to contribute to the mechanisms underlying the development of atherosclerosis.",
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T1 - Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors

AU - Alberti, L.

AU - Gilardini, L.

AU - Zulian, A.

AU - Micheletto, G.

AU - Peri, G.

AU - Doni, A.

AU - Mantovani, A.

AU - Invitti, C.

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N2 - Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4 ± 8.15 yr) and 10 normal weight subjects (43.7 ± 11.07 yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor α (TNFα) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNFα and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNFα and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only (p = 0.05). CD68 and PTX3 expressions were correlated with each other in SAT but not in VAT. After adjustment for age and sex, VAT-PTX3 expression and release were correlated with VAT-TNFα expression (p <0.01 for both) and with LDL/HDL ratio (p <0.01 and p <0.001). VAT-PTX3 expression was also correlated with BMI, triglycerides, CRP, fibrinogen and adiponectin (p <0.05 for all). In the multivariate analysis with VAT-PTX3 RNA levels as dependent variable, LDL/HDL ratio and fibrinogen remained independently associated with VAT-PTX3 expression (p <0.01 for both). These associations were not seen within SAT. Conclusions: Human adipose tissue expresses and releases PTX3 likely under TNFα control. VAT production of PTX3 seems to contribute to the mechanisms underlying the development of atherosclerosis.

AB - Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4 ± 8.15 yr) and 10 normal weight subjects (43.7 ± 11.07 yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor α (TNFα) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNFα and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNFα and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only (p = 0.05). CD68 and PTX3 expressions were correlated with each other in SAT but not in VAT. After adjustment for age and sex, VAT-PTX3 expression and release were correlated with VAT-TNFα expression (p <0.01 for both) and with LDL/HDL ratio (p <0.01 and p <0.001). VAT-PTX3 expression was also correlated with BMI, triglycerides, CRP, fibrinogen and adiponectin (p <0.05 for all). In the multivariate analysis with VAT-PTX3 RNA levels as dependent variable, LDL/HDL ratio and fibrinogen remained independently associated with VAT-PTX3 expression (p <0.01 for both). These associations were not seen within SAT. Conclusions: Human adipose tissue expresses and releases PTX3 likely under TNFα control. VAT production of PTX3 seems to contribute to the mechanisms underlying the development of atherosclerosis.

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KW - Fibrinogen

KW - HDL

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