Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies

B. Falini, H. Stein, S. Pileri, S. Canino, R. Farabbi, M. F. Martelli, F. Grignani, M. Fagioli, O. Minelli, C. Ciani, L. Flenghi

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The aim of this study was to elucidate the origin of Hodgkin's and Reed-Sternberg cells. Lymph node cytospins and frozen sections from 20 cases of Hodgkin's disease of different histological subtypes were immunostained by the immuno-alkaline phosphatase technique using a panel of monoclonal antibodies. As expected, the Hodgkin's and Reed-Sternberg cells of all cases were positive for the CD30 (Ki-1), CD15 (hapten X) and CD25 (Tac) antigens. In eight cases, a variable percentage of typical Hodgkin's and Reed-Sternberg cells showed a clear-cut cytoplasmic and/or surface positivity for the T-cell-associated antigens CD3, CD5, CD6 and CD4 (seven cases) or CD8 (one case), but consistently lacked B-cell and macrophage-associated markers. The best visualization of T-cell antigens was obtained in cytocentrifuge preparations and in areas of lymph node frozen sections that had been infiltrated by clusters of Hodgkin's and Reed-Sternberg cells. In two cases of Hodgkin's disease (nodular sclerosis, mixed cellularity) the neoplastic cells weakly expressed the B-cell antigens CD19 and CD22, but not T-cell or macrophage-associated markers. In 10 cases. Hodgkin's and Reed-Sternberg cells were negative for all the lymphoid- and macrophage-associated antigens. These results suggest a lymphoid (either T or B) rather than histiocytic origin for the Hodgkin's and Reed-Sternberg cells in a number of Hodgkin's disease cases.

Original languageEnglish
Pages (from-to)1229-1242
Number of pages14
JournalHistopathology
Volume11
Issue number12
Publication statusPublished - 1987

Fingerprint

Reed-Sternberg Cells
Hodgkin Disease
Lymph Nodes
Monoclonal Antibodies
Antigens
Macrophages
Frozen Sections
T-Lymphocytes
CD19 Antigens
CD5 Antigens
CD15 Antigens
Interleukin-2 Receptor alpha Subunit
CD3 Antigens
CD4 Antigens
Viral Tumor Antigens
Sclerosis
Alkaline Phosphatase
B-Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. / Falini, B.; Stein, H.; Pileri, S.; Canino, S.; Farabbi, R.; Martelli, M. F.; Grignani, F.; Fagioli, M.; Minelli, O.; Ciani, C.; Flenghi, L.

In: Histopathology, Vol. 11, No. 12, 1987, p. 1229-1242.

Research output: Contribution to journalArticle

Falini, B, Stein, H, Pileri, S, Canino, S, Farabbi, R, Martelli, MF, Grignani, F, Fagioli, M, Minelli, O, Ciani, C & Flenghi, L 1987, 'Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies', Histopathology, vol. 11, no. 12, pp. 1229-1242.
Falini, B. ; Stein, H. ; Pileri, S. ; Canino, S. ; Farabbi, R. ; Martelli, M. F. ; Grignani, F. ; Fagioli, M. ; Minelli, O. ; Ciani, C. ; Flenghi, L. / Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies. In: Histopathology. 1987 ; Vol. 11, No. 12. pp. 1229-1242.
@article{2c1b6f399cc849d1a7a834df5a189fb2,
title = "Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies",
abstract = "The aim of this study was to elucidate the origin of Hodgkin's and Reed-Sternberg cells. Lymph node cytospins and frozen sections from 20 cases of Hodgkin's disease of different histological subtypes were immunostained by the immuno-alkaline phosphatase technique using a panel of monoclonal antibodies. As expected, the Hodgkin's and Reed-Sternberg cells of all cases were positive for the CD30 (Ki-1), CD15 (hapten X) and CD25 (Tac) antigens. In eight cases, a variable percentage of typical Hodgkin's and Reed-Sternberg cells showed a clear-cut cytoplasmic and/or surface positivity for the T-cell-associated antigens CD3, CD5, CD6 and CD4 (seven cases) or CD8 (one case), but consistently lacked B-cell and macrophage-associated markers. The best visualization of T-cell antigens was obtained in cytocentrifuge preparations and in areas of lymph node frozen sections that had been infiltrated by clusters of Hodgkin's and Reed-Sternberg cells. In two cases of Hodgkin's disease (nodular sclerosis, mixed cellularity) the neoplastic cells weakly expressed the B-cell antigens CD19 and CD22, but not T-cell or macrophage-associated markers. In 10 cases. Hodgkin's and Reed-Sternberg cells were negative for all the lymphoid- and macrophage-associated antigens. These results suggest a lymphoid (either T or B) rather than histiocytic origin for the Hodgkin's and Reed-Sternberg cells in a number of Hodgkin's disease cases.",
author = "B. Falini and H. Stein and S. Pileri and S. Canino and R. Farabbi and Martelli, {M. F.} and F. Grignani and M. Fagioli and O. Minelli and C. Ciani and L. Flenghi",
year = "1987",
language = "English",
volume = "11",
pages = "1229--1242",
journal = "Histopathology",
issn = "0309-0167",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Expression of lymphoid-associated antigens on Hodgkin's and Reed-Sternberg cells of Hodgkin's disease. An immunocytochemical study on lymph node cytospins using monoclonal antibodies

AU - Falini, B.

AU - Stein, H.

AU - Pileri, S.

AU - Canino, S.

AU - Farabbi, R.

AU - Martelli, M. F.

AU - Grignani, F.

AU - Fagioli, M.

AU - Minelli, O.

AU - Ciani, C.

AU - Flenghi, L.

PY - 1987

Y1 - 1987

N2 - The aim of this study was to elucidate the origin of Hodgkin's and Reed-Sternberg cells. Lymph node cytospins and frozen sections from 20 cases of Hodgkin's disease of different histological subtypes were immunostained by the immuno-alkaline phosphatase technique using a panel of monoclonal antibodies. As expected, the Hodgkin's and Reed-Sternberg cells of all cases were positive for the CD30 (Ki-1), CD15 (hapten X) and CD25 (Tac) antigens. In eight cases, a variable percentage of typical Hodgkin's and Reed-Sternberg cells showed a clear-cut cytoplasmic and/or surface positivity for the T-cell-associated antigens CD3, CD5, CD6 and CD4 (seven cases) or CD8 (one case), but consistently lacked B-cell and macrophage-associated markers. The best visualization of T-cell antigens was obtained in cytocentrifuge preparations and in areas of lymph node frozen sections that had been infiltrated by clusters of Hodgkin's and Reed-Sternberg cells. In two cases of Hodgkin's disease (nodular sclerosis, mixed cellularity) the neoplastic cells weakly expressed the B-cell antigens CD19 and CD22, but not T-cell or macrophage-associated markers. In 10 cases. Hodgkin's and Reed-Sternberg cells were negative for all the lymphoid- and macrophage-associated antigens. These results suggest a lymphoid (either T or B) rather than histiocytic origin for the Hodgkin's and Reed-Sternberg cells in a number of Hodgkin's disease cases.

AB - The aim of this study was to elucidate the origin of Hodgkin's and Reed-Sternberg cells. Lymph node cytospins and frozen sections from 20 cases of Hodgkin's disease of different histological subtypes were immunostained by the immuno-alkaline phosphatase technique using a panel of monoclonal antibodies. As expected, the Hodgkin's and Reed-Sternberg cells of all cases were positive for the CD30 (Ki-1), CD15 (hapten X) and CD25 (Tac) antigens. In eight cases, a variable percentage of typical Hodgkin's and Reed-Sternberg cells showed a clear-cut cytoplasmic and/or surface positivity for the T-cell-associated antigens CD3, CD5, CD6 and CD4 (seven cases) or CD8 (one case), but consistently lacked B-cell and macrophage-associated markers. The best visualization of T-cell antigens was obtained in cytocentrifuge preparations and in areas of lymph node frozen sections that had been infiltrated by clusters of Hodgkin's and Reed-Sternberg cells. In two cases of Hodgkin's disease (nodular sclerosis, mixed cellularity) the neoplastic cells weakly expressed the B-cell antigens CD19 and CD22, but not T-cell or macrophage-associated markers. In 10 cases. Hodgkin's and Reed-Sternberg cells were negative for all the lymphoid- and macrophage-associated antigens. These results suggest a lymphoid (either T or B) rather than histiocytic origin for the Hodgkin's and Reed-Sternberg cells in a number of Hodgkin's disease cases.

UR - http://www.scopus.com/inward/record.url?scp=0023621763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023621763&partnerID=8YFLogxK

M3 - Article

C2 - 2831131

AN - SCOPUS:0023621763

VL - 11

SP - 1229

EP - 1242

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 12

ER -