Expression of metabotropic glutamate receptors in murine thymocytes and thymic stromal cells

Marianna Storto, Ugo De Grazia, Giuseppe Battaglia, Maria Pia Felli, Marella Maroder, Alberto Gulino, Giuseppe Ragona, Ferdinando Nicoletti, Isabella Screpanti, Luigi Frati, Antonella Calogero

Research output: Contribution to journalArticle

Abstract

RT-PCR combined with immunoblotting showed the expression of group-I (mGlu1 and 5) and group-II (mGlu2 and 3) metabotropic glutamate receptors in whole mouse thymus, isolated thymocytes and TC-1S thymic stromal cell line. Cytofluorimetric analysis showed that mGlu-5 receptors were absent in CD4- /CD8- but present in more mature CD4+ CD8+ and CD4+ CD8- thymocytes. mGlu-1a receptors showed an opposite pattern of expression with respect to mGlu5, whereas mGlu2/3 receptor expression did not differ between double negative and double positive cells. mGlu receptors expressed in both thymic cell components were functional, as indicated by measurements of polyphosphoinositide hydrolysis or cAMP formation. These data suggest a possible role for mGlu receptor signalling in the thymus. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalJournal of Neuroimmunology
Volume109
Issue number2
DOIs
Publication statusPublished - Sep 22 2000

Keywords

  • Metabotropic glutamate receptors
  • TC-1S cells
  • Thymocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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