Expression of natriuretic peptide in ventricular myocardium of failing human hearts and its correlation with the severity of clinical and hemodynamic impairment

Eloisa Arbustini, Angela Pucci, Maurizia Grasso, Marta Diegoli, Roberto Pozzi, Antonello Gavazzi, Gabriella Graziano, Carlo Campana, Claudio Goggi, Luigi Martinelli, Enrico Silini, Giuseppe Specchia, Mario Vigano, Enrico Solcia

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Abstract

Atrial natriuretic peptide (ANP) was immunohistochemically investigated in (1) right ventricular endomyocardial biopsy specimens from 87 apparently healthy donor hearts taken from victims of cerebral accidents; (2) 1 normal heart not suitable for transplantation (HBsAg carrier); (3) right ventricular endomyocardial biopsy specimens from 151 patients with dilated cardiomyopathy (DC); and (4) 57 explanted hearts, 26 with DC and 31 with ischemic heart disease. No ANP immunoreactivity was found in normal ventricles. Failing hearts showed ventricular positivity in 31% of the DC biopsy series, in 61% of the left ventricles, and in 30% of the right ventricles of the explanted heart series. An endoepicardial gradient was observed, because ANP positivity was greater and more extensive in the subendocardial layers. Ultrastructural studies were performed on biopsy specimens from 10 normal hearts and 132 DC biopsy samples. No ANP-storing granules were found in biopsy samples of normal ventricles, whereas ANP granules were seen in 15 of 132 (11.4%) DC cases. In parallel imiminoblotting, investigation showed the same 13 kDa band protein in 1 normal atrium as well as in 8 failing atria and ventricles. ANP immunoreactivity was positively correlated with higher New York Heart Association functional classes as well as with higher left ventricular end-diastolic pressure (p <0.005), end-diastolic volume (p <0.005) and enddiastolic volume index (p <0.005). In conclusion, apparently healthy ventricles do not show ANP immunoreactivity, whereas failing ventricles do. ANP expression seems to be independent of the underlying disease, but positively related to the clinical status and the degree of left ventricular impairment and dilatation.

Original languageEnglish
Pages (from-to)973-980
Number of pages8
JournalThe American Journal of Cardiology
Volume66
Issue number12
DOIs
Publication statusPublished - Oct 15 1990

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Natriuretic Peptides
Atrial Natriuretic Factor
Myocardium
Hemodynamics
Dilated Cardiomyopathy
Biopsy
Heart Ventricles
Hepatitis B Surface Antigens
Accidents
Myocardial Ischemia
Dilatation
Transplantation
Tissue Donors
Blood Pressure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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Expression of natriuretic peptide in ventricular myocardium of failing human hearts and its correlation with the severity of clinical and hemodynamic impairment. / Arbustini, Eloisa; Pucci, Angela; Grasso, Maurizia; Diegoli, Marta; Pozzi, Roberto; Gavazzi, Antonello; Graziano, Gabriella; Campana, Carlo; Goggi, Claudio; Martinelli, Luigi; Silini, Enrico; Specchia, Giuseppe; Vigano, Mario; Solcia, Enrico.

In: The American Journal of Cardiology, Vol. 66, No. 12, 15.10.1990, p. 973-980.

Research output: Contribution to journalArticle

Arbustini, Eloisa ; Pucci, Angela ; Grasso, Maurizia ; Diegoli, Marta ; Pozzi, Roberto ; Gavazzi, Antonello ; Graziano, Gabriella ; Campana, Carlo ; Goggi, Claudio ; Martinelli, Luigi ; Silini, Enrico ; Specchia, Giuseppe ; Vigano, Mario ; Solcia, Enrico. / Expression of natriuretic peptide in ventricular myocardium of failing human hearts and its correlation with the severity of clinical and hemodynamic impairment. In: The American Journal of Cardiology. 1990 ; Vol. 66, No. 12. pp. 973-980.
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abstract = "Atrial natriuretic peptide (ANP) was immunohistochemically investigated in (1) right ventricular endomyocardial biopsy specimens from 87 apparently healthy donor hearts taken from victims of cerebral accidents; (2) 1 normal heart not suitable for transplantation (HBsAg carrier); (3) right ventricular endomyocardial biopsy specimens from 151 patients with dilated cardiomyopathy (DC); and (4) 57 explanted hearts, 26 with DC and 31 with ischemic heart disease. No ANP immunoreactivity was found in normal ventricles. Failing hearts showed ventricular positivity in 31{\%} of the DC biopsy series, in 61{\%} of the left ventricles, and in 30{\%} of the right ventricles of the explanted heart series. An endoepicardial gradient was observed, because ANP positivity was greater and more extensive in the subendocardial layers. Ultrastructural studies were performed on biopsy specimens from 10 normal hearts and 132 DC biopsy samples. No ANP-storing granules were found in biopsy samples of normal ventricles, whereas ANP granules were seen in 15 of 132 (11.4{\%}) DC cases. In parallel imiminoblotting, investigation showed the same 13 kDa band protein in 1 normal atrium as well as in 8 failing atria and ventricles. ANP immunoreactivity was positively correlated with higher New York Heart Association functional classes as well as with higher left ventricular end-diastolic pressure (p <0.005), end-diastolic volume (p <0.005) and enddiastolic volume index (p <0.005). In conclusion, apparently healthy ventricles do not show ANP immunoreactivity, whereas failing ventricles do. ANP expression seems to be independent of the underlying disease, but positively related to the clinical status and the degree of left ventricular impairment and dilatation.",
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T1 - Expression of natriuretic peptide in ventricular myocardium of failing human hearts and its correlation with the severity of clinical and hemodynamic impairment

AU - Arbustini, Eloisa

AU - Pucci, Angela

AU - Grasso, Maurizia

AU - Diegoli, Marta

AU - Pozzi, Roberto

AU - Gavazzi, Antonello

AU - Graziano, Gabriella

AU - Campana, Carlo

AU - Goggi, Claudio

AU - Martinelli, Luigi

AU - Silini, Enrico

AU - Specchia, Giuseppe

AU - Vigano, Mario

AU - Solcia, Enrico

PY - 1990/10/15

Y1 - 1990/10/15

N2 - Atrial natriuretic peptide (ANP) was immunohistochemically investigated in (1) right ventricular endomyocardial biopsy specimens from 87 apparently healthy donor hearts taken from victims of cerebral accidents; (2) 1 normal heart not suitable for transplantation (HBsAg carrier); (3) right ventricular endomyocardial biopsy specimens from 151 patients with dilated cardiomyopathy (DC); and (4) 57 explanted hearts, 26 with DC and 31 with ischemic heart disease. No ANP immunoreactivity was found in normal ventricles. Failing hearts showed ventricular positivity in 31% of the DC biopsy series, in 61% of the left ventricles, and in 30% of the right ventricles of the explanted heart series. An endoepicardial gradient was observed, because ANP positivity was greater and more extensive in the subendocardial layers. Ultrastructural studies were performed on biopsy specimens from 10 normal hearts and 132 DC biopsy samples. No ANP-storing granules were found in biopsy samples of normal ventricles, whereas ANP granules were seen in 15 of 132 (11.4%) DC cases. In parallel imiminoblotting, investigation showed the same 13 kDa band protein in 1 normal atrium as well as in 8 failing atria and ventricles. ANP immunoreactivity was positively correlated with higher New York Heart Association functional classes as well as with higher left ventricular end-diastolic pressure (p <0.005), end-diastolic volume (p <0.005) and enddiastolic volume index (p <0.005). In conclusion, apparently healthy ventricles do not show ANP immunoreactivity, whereas failing ventricles do. ANP expression seems to be independent of the underlying disease, but positively related to the clinical status and the degree of left ventricular impairment and dilatation.

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