Expression of natriuretic peptide receptors in human adipose and other tissues

R. Sarzani, P. Dessì-Fulgheri, V. M. Paci, E. Espinosa, A. Rappelli

Research output: Contribution to journalArticlepeer-review


To characterize natriuretic peptide receptor (NPr) gene expression in human tissues, we cloned portions of the cDNAs codifying for NPr with guanylyl cyclase activity (NPr-A and NPr-B) and without guanylyl cyclase activity (NPr-C). Total RNA was extracted from samples taken at surgery from normal human tissues. NPr-A and NPr-B cDNAs obtained from lung as well as NPr-C cDNA obtained from renal cortex were cloned, characterized, and used for comparative Northern analysis. NPr-A mRNA (~4 kb) was most abundant in adipose tissue (8 patients) independently on the site of sampling, whereas it was ~2.5-fold and 5-fold less abundant, respectively, in kidney (either renal cortex or papilla from 3 patients) and adrenal (4 patients), known target tissues of natriuretic peptides. NPr-C mRNAs (~7.7 and 6.8 kb) had a similar tissue distribution but the highest levels were found in renal tissue and only very low expression levels were found in adrenals (~20-fold lower than renal cortex). The ratio of NPrA Versus NPr-C mRNA levels were highest in adrenal and lowest in renal tissue. NPr-B mRNA (~4 kb), which encodes the receptor for the C-type natriuretic peptide, had a different and wide tissue distribution, including expression in ileum and liver, with the highest levels in venous and prostatic tissue. These results indicate that, in humans, different patterns of NPr expression with different NPrA/NPr-C mRNA level ratios, are present in known target tissues of natriuretic peptides. 'Non-classic' target tissues, such as the adipose one, maximally expressed NPr-A and also NPr-C, suggesting that natriuretic peptides may have wider functional activities than those previously demonstrated.

Original languageEnglish
Pages (from-to)581-585
Number of pages5
JournalJournal of Endocrinological Investigation
Issue number9
Publication statusPublished - Oct 1996


  • Adipose tissue
  • Atrial natriuretic peptide
  • Clearance receptor
  • Gene expression
  • Guanylyl cyclase
  • Natriuretic peptides
  • Receptors

ASJC Scopus subject areas

  • Endocrinology


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