Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes

Fiohenzo Conti, Silvia DeBiasi, Rea Minelli, Marcello Melone

Research output: Contribution to journalArticle

Abstract

Ionotropic glutamate (Glu) receptors of the N-methyl-D- aspartate type (NMDA) play a fundamental role in many cortical functions. Native NMDA receptors are composed of a heteromeric assembly of different subunits belonging to two classes: NMDAR1 (NR1) and NMDAR2 (NR2). To date, NMDA receptors are believed to be expressed only in neurons, although electrophysiological and in situ hybridization studies have suggested that this class of Glu receptors might be also expressed by some astrocytes. In this study, we have investigated in the cerebral cortex of adult rats the presence of astrocytes expressing NR1 and NR2A/B subunits by immunocytochemistry with specific antibodies, and we show that some distal astrocytic processes, but only rarely astrocytic cell bodies, contain immunoreaction product indicative of NR1 and NR2A/B expression. These findings suggest that at least part of the role NMDA has in cortical functions might depend on the activation of astrocytic NMDA receptors; the subcellular localization of NR1 and NR2A/B subunits in distal processes suggests that NMDA receptors contribute to monitoring Glu levels in the extracellular space.

Original languageEnglish
Pages (from-to)254-258
Number of pages5
JournalGLIA
Volume17
Issue number3
DOIs
Publication statusPublished - Jul 1996

Fingerprint

N-Methyl-D-Aspartate Receptors
Astrocytes
N-Methylaspartate
Ionotropic Glutamate Receptors
Glutamate Receptors
Extracellular Space
Cerebral Cortex
In Situ Hybridization
Glutamic Acid
Immunohistochemistry
Neurons
NMDA receptor A1
Antibodies

Keywords

  • Glutamate
  • ionotropic receptors
  • neuron-astrocyte communication

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes. / Conti, Fiohenzo; DeBiasi, Silvia; Minelli, Rea; Melone, Marcello.

In: GLIA, Vol. 17, No. 3, 07.1996, p. 254-258.

Research output: Contribution to journalArticle

Conti, Fiohenzo ; DeBiasi, Silvia ; Minelli, Rea ; Melone, Marcello. / Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes. In: GLIA. 1996 ; Vol. 17, No. 3. pp. 254-258.
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