Expression of p34(cdc)2 and cyclins A and B compared to other proliferative features of non-Hodgkin's lymphomas: A multivariate cluster analysis

Lorenzo Leoncini, Antonio Cossu, Tiziana Megha, Cristiana Bellan, Stefano Lazzi, Pietro Luzi, Piero Tosi, Paolo Barbini, Gabriele Cevenini, Stefano Pileri, Antonio Giordano, Rainer Kraft, Jean A. Laissue, Hans Cottier

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In view of recent knowledge on proteins regulating the cell cycle, we re-evaluated proliferative features of 98 diffusely growing non-Hodgkin's lymphomas. The combined use of 5 proliferation-associated variables (mitotic indices and percentages of Ki-673, p3(4cdc)2+, cyclin A+ and cyclin B+ cells) and their entry into a multivariate cluster analysis separated, without overlaps, the entire cohort into 3 groups (clusters) with (1) low, (2) intermediate and (3) high proliferative activity. Conversely, bivariate plots exposed considerable cluster overlaps. Multivariate stepwise discriminant analysis of all cases revealed a decreasing order of discriminant power for% Ki-67+ cells > % p34(cdc)2+ cells > mitotic index > % cyclin A+ cells > % cyclin B+ cells. The combined use of 2 variables only, mitotic index and % p34(cdc)2+ cells, allowed a clear-cut separation of clusters 2 and 3. In bivariate plots, correlations were best between % Ki- 67+ cells and % cyclin A+ cells and between mitotic indices and % cyclin B+ cells. Except for chronic lymphocytic leukemias, immunocytomas and marginal zone lymphomas (all in cluster 1), individual lymphoma entities were distributed among at least 2 clusters. There was, however, a marked preponderance of mantle cell lymphomas and diffuse follicular center lymphomas in cluster 1 and of diffuse large B-cell lymphomas and peripheral T-cell lymphomas in cluster 2. Anaplastic large-cell lymphomas predominated in cluster 3 and responded best to therapy.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalInternational Journal of Cancer
Issue number2
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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