Expression of polycystin-1 C-terminal fragment enhances the ATP-induced Ca2+ release in human kidney cells

Gianluca Aguiari, Michelangelo Campanella, Elisa Manzati, Paolo Pinton, Manuela Banzi, Sabrina Moretti, Roberta Piva, Rosario Rizzuto, Laura Del Senno

Research output: Contribution to journalArticle

Abstract

Polycystin-1 (PC1) is a membrane protein expressed in tubular epithelia of developing kidneys and in other ductal structures. Recent studies indicate this protein to be putatively important in regulating intracellular Ca2+ levels in various cell types, but little evidence exists for kidney epithelial cells. Here we examined the role of the PC1 cytoplasmic tail on the activity of store operated Ca2+ channels in human kidney epithelial HEK-293 cell line. Cells were transiently transfected with chimeric proteins containing 1-226 or 26-226 aa of the PC1 cytoplasmic tail fused to the transmembrane domain of the human Trk-A receptor: TrkPC1 wild-type and control Trk truncated peptides were expressed at comparable levels and localized at the plasma membrane. Ca2+ measurements were performed in cells co-transfected with PC1 chimeras and the cytoplasmic Ca2+-sensitive photoprotein aequorin, upon activation of the phosphoinositide pathway by ATP, that, via purinoceptors, is coupled to the release of Ca2+ from intracellular stores. The expression of TrkPC1 peptide, but not of its truncated form, enhanced the ATP-evoked cytosolic Ca2+ concentrations. When Ca2+ assays were performed in HeLa cells characterized by Ca2+ stores greater than those of HEK-293 cells, the histamine-evoked cytosolic Ca2+ increase was enhanced by TrkPC1 expression, even in absence of external Ca2+. These observations indicate that the C-terminal tail of PC1 in kidney and other epithelial cells upregulates a Ca2+ channel activity also involved in the release of intracellular stores.

Original languageEnglish
Pages (from-to)657-664
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number3
DOIs
Publication statusPublished - Feb 14 2003

Keywords

  • Epithelial cells
  • HEK 293 cells
  • HeLa cells
  • Intracellular Ca
  • Polycystin-1
  • Polycystin-2
  • Protein expression
  • TrkPC1 chimeric receptors

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Aguiari, G., Campanella, M., Manzati, E., Pinton, P., Banzi, M., Moretti, S., Piva, R., Rizzuto, R., & Del Senno, L. (2003). Expression of polycystin-1 C-terminal fragment enhances the ATP-induced Ca2+ release in human kidney cells. Biochemical and Biophysical Research Communications, 301(3), 657-664. https://doi.org/10.1016/S0006-291X(02)03011-5