Expression of protein kinase C-alpha (PKC-α) and MYCN mRNAs in human neuroblastoma cells and modulation during morphological differentiation induced by retinoic acid

Gian Paolo Tonini, Maria Teresa Parodi, Daniela Di Martino, Luigi Varesio

Research output: Contribution to journalArticle

Abstract

It is known that PKC is differently expressed in brain and the peripheral nervous system and is involved in cellular differentiation. We have analyzed 9 human neuronal-derived crest-cell lines for PKC-α mRNA. Seven out of nine expressed 9.0 kb and 4.0 kb PKC-α mRNAs but three had high level of 9.0 kb transcription. The different expression of the two messenger RNAs may result from alternative splicing and a different degree of cell maturation. The same cell lines were studied for MYCN gene expression. A possible relation between the two genes is discussed. One cell line expressing high levels of both PKC-α mRNA was treated with 10-1 M retinoic acid (RA). The expression of both messenger RNAs was suppressed when the cells achieved a morphological differentiation and showed neurite-like processes. A decrease of PKC-α gene expression was associated to down regulation of MYCN mRNA. These preliminary results suggest that PKC suppression of PKC-α mRNA is associated with reversion of the malignant phenotype.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalFEBS Letters
Volume280
Issue number2
DOIs
Publication statusPublished - Mar 25 1991

Keywords

  • Gene expression
  • Human neuroblastoma
  • Neuronal differentiation
  • PKC-α
  • Retinoic acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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