Expression of retroviral sequences and oncogenes in murine hepatocellular tumors

T. A. Dragani, G. Manenti, G. della Porta, S. Gattoni-Celli, I. B. Weinstein

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of endogenous retroviral sequences and of three cellular oncogenes was examined in three hepatocellular adenomas and in four carcinomas induced in male C57BL/5JDp x C3Hf/Dp F1 (hereafter called B6C3F1) mice by a single dose of nitrosodiethylamine, in five carcinomas that arose spontaneously in male C3Hf mice, and in the livers of normal age-matched control mice. In all of these adenomas and carcinomas, there was increased expression of Moloney murine leukemia virus- and intracisternal A particle-related sequences. The retrovirus-like VL30 sequence was expressed at significant levels in the normal liver of these mice and increased expression of this sequence was found in only 4 of the 12 tumors examined. Expression of endogenous mouse mammary tumor virus-related sequences was not detected in the normal livers or in any of the liver tumors. With respect to cellular oncogenes, increased expression of c-myc was seen in all of the B6C3F1 tumors. Two of five normal liver samples and all of the C3Hf mice displayed significant expression of c-myc. There was a light increase in expression of c-Ha-ras in some of the tumors. Increased expression of c-fos was found in only 1 of the 12 tumors. Taken together, these studies indicate that both carcinogen-induced and spontaneous liver tumor formation in mice is associated with abnormalities in the expression of endogenous retrovirus-related DNA sequences and also specific cellular oncogenes.

Original languageEnglish
Pages (from-to)1915-1919
Number of pages5
JournalCancer Research
Volume46
Issue number4 II
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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