Expression of runtB is modulated during chondrocyte differentiation

Patrizio Castagnola, Massimo Gennari, Alessia Gaggero, Fabio Rossi, Antonio Daga, Maria Teresa Corsetti, Franco Calabi, Ranieri Cancedda

Research output: Contribution to journalArticle

Abstract

The runt locus in Drosophila encodes a nuclear protein involved in embryo segmentation, sex determination/X dosage compensation, and neurogenesis. runt homologues have been identified in higher vertebrates. The encoded proteins share a domain of 128 amino acids called the runt domain. It has been reported that this domain mediates DNA binding and heterodimerization. Here, we analyze runtB expression during chondrocyte differentiation 'in vitro' and 'in vivo.' We have first isolated, from a chondrocyte library, a cDNA clone coding for a runtB chicken homologue and containing a complete open reading frame. The predicted protein product is 84% identical to the mouse PEBP2αB2 isoform. By RT-PCR analysis we have also cloned the chicken cDNA fragment coding for ΔαB2, the exon sequence included in the B1 isoform mRNA. On Northern blot analysis of cultured chondrocytes, runtB mRNA levels increase dramatically with the transition from stage 0 (dedifferentiated) to stages I and II (hypertrophic chondrocytes). Moreover, runt polypeptides were demonstrated in chondrocytes both in vivo and in vitro. These results suggest that runt plays a role in chondrogenic differentiation.

Original languageEnglish
Pages (from-to)215-226
Number of pages12
JournalExperimental Cell Research
Volume223
Issue number2
DOIs
Publication statusPublished - Mar 15 1996

Fingerprint

Chondrocytes
Chickens
RNA Isoforms
Neurogenesis
Nuclear Proteins
Gene Library
Northern Blotting
Open Reading Frames
Drosophila
Vertebrates
Exons
Protein Isoforms
Proteins
Embryonic Structures
Complementary DNA
Clone Cells
Amino Acids
Polymerase Chain Reaction
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression of runtB is modulated during chondrocyte differentiation. / Castagnola, Patrizio; Gennari, Massimo; Gaggero, Alessia; Rossi, Fabio; Daga, Antonio; Corsetti, Maria Teresa; Calabi, Franco; Cancedda, Ranieri.

In: Experimental Cell Research, Vol. 223, No. 2, 15.03.1996, p. 215-226.

Research output: Contribution to journalArticle

Castagnola, P, Gennari, M, Gaggero, A, Rossi, F, Daga, A, Corsetti, MT, Calabi, F & Cancedda, R 1996, 'Expression of runtB is modulated during chondrocyte differentiation', Experimental Cell Research, vol. 223, no. 2, pp. 215-226. https://doi.org/10.1006/excr.1996.0075
Castagnola, Patrizio ; Gennari, Massimo ; Gaggero, Alessia ; Rossi, Fabio ; Daga, Antonio ; Corsetti, Maria Teresa ; Calabi, Franco ; Cancedda, Ranieri. / Expression of runtB is modulated during chondrocyte differentiation. In: Experimental Cell Research. 1996 ; Vol. 223, No. 2. pp. 215-226.
@article{e18ca382beb34a51a9dd06a4bcfee2f9,
title = "Expression of runtB is modulated during chondrocyte differentiation",
abstract = "The runt locus in Drosophila encodes a nuclear protein involved in embryo segmentation, sex determination/X dosage compensation, and neurogenesis. runt homologues have been identified in higher vertebrates. The encoded proteins share a domain of 128 amino acids called the runt domain. It has been reported that this domain mediates DNA binding and heterodimerization. Here, we analyze runtB expression during chondrocyte differentiation 'in vitro' and 'in vivo.' We have first isolated, from a chondrocyte library, a cDNA clone coding for a runtB chicken homologue and containing a complete open reading frame. The predicted protein product is 84{\%} identical to the mouse PEBP2αB2 isoform. By RT-PCR analysis we have also cloned the chicken cDNA fragment coding for ΔαB2, the exon sequence included in the B1 isoform mRNA. On Northern blot analysis of cultured chondrocytes, runtB mRNA levels increase dramatically with the transition from stage 0 (dedifferentiated) to stages I and II (hypertrophic chondrocytes). Moreover, runt polypeptides were demonstrated in chondrocytes both in vivo and in vitro. These results suggest that runt plays a role in chondrogenic differentiation.",
author = "Patrizio Castagnola and Massimo Gennari and Alessia Gaggero and Fabio Rossi and Antonio Daga and Corsetti, {Maria Teresa} and Franco Calabi and Ranieri Cancedda",
year = "1996",
month = "3",
day = "15",
doi = "10.1006/excr.1996.0075",
language = "English",
volume = "223",
pages = "215--226",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of runtB is modulated during chondrocyte differentiation

AU - Castagnola, Patrizio

AU - Gennari, Massimo

AU - Gaggero, Alessia

AU - Rossi, Fabio

AU - Daga, Antonio

AU - Corsetti, Maria Teresa

AU - Calabi, Franco

AU - Cancedda, Ranieri

PY - 1996/3/15

Y1 - 1996/3/15

N2 - The runt locus in Drosophila encodes a nuclear protein involved in embryo segmentation, sex determination/X dosage compensation, and neurogenesis. runt homologues have been identified in higher vertebrates. The encoded proteins share a domain of 128 amino acids called the runt domain. It has been reported that this domain mediates DNA binding and heterodimerization. Here, we analyze runtB expression during chondrocyte differentiation 'in vitro' and 'in vivo.' We have first isolated, from a chondrocyte library, a cDNA clone coding for a runtB chicken homologue and containing a complete open reading frame. The predicted protein product is 84% identical to the mouse PEBP2αB2 isoform. By RT-PCR analysis we have also cloned the chicken cDNA fragment coding for ΔαB2, the exon sequence included in the B1 isoform mRNA. On Northern blot analysis of cultured chondrocytes, runtB mRNA levels increase dramatically with the transition from stage 0 (dedifferentiated) to stages I and II (hypertrophic chondrocytes). Moreover, runt polypeptides were demonstrated in chondrocytes both in vivo and in vitro. These results suggest that runt plays a role in chondrogenic differentiation.

AB - The runt locus in Drosophila encodes a nuclear protein involved in embryo segmentation, sex determination/X dosage compensation, and neurogenesis. runt homologues have been identified in higher vertebrates. The encoded proteins share a domain of 128 amino acids called the runt domain. It has been reported that this domain mediates DNA binding and heterodimerization. Here, we analyze runtB expression during chondrocyte differentiation 'in vitro' and 'in vivo.' We have first isolated, from a chondrocyte library, a cDNA clone coding for a runtB chicken homologue and containing a complete open reading frame. The predicted protein product is 84% identical to the mouse PEBP2αB2 isoform. By RT-PCR analysis we have also cloned the chicken cDNA fragment coding for ΔαB2, the exon sequence included in the B1 isoform mRNA. On Northern blot analysis of cultured chondrocytes, runtB mRNA levels increase dramatically with the transition from stage 0 (dedifferentiated) to stages I and II (hypertrophic chondrocytes). Moreover, runt polypeptides were demonstrated in chondrocytes both in vivo and in vitro. These results suggest that runt plays a role in chondrogenic differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0029964162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029964162&partnerID=8YFLogxK

U2 - 10.1006/excr.1996.0075

DO - 10.1006/excr.1996.0075

M3 - Article

C2 - 8601397

AN - SCOPUS:0029964162

VL - 223

SP - 215

EP - 226

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -