Expression of serotonin receptors 2B and 4 in human prostate cancer tissue and effects of their antagonists on prostate cancer cell lines

N. Dizeyi, A. Bjartell, P. Hedlund, K. A. Taskén, V. Gadaleanu, P. A. Abrahamsson

Research output: Contribution to journalArticle

Abstract

Objectives: Overexpression of receptors to neuroendocrine (NE) cell products has been suggested to contribute to development of hormone-refractory prostate cancer (HRPC). In this study, we evaluated the expression of 5-HTR2B and 5-HTR4 in HRPC, and the effects of their antagonist on PC cell line growth. Methods: Proteins and mRNA expression was determined by immunohistochemistry, western blot and RT-PCR. Growth inhibition of PC cell lines was determined in vitro using ELISA-BrdU proliferation assay and cell cycle was evaluated by flow cytometry. Results: Immunostaining of 5-HTR2B was observed in low-grade and high-grade tumours, PIN and BPH cells, and in vascular endothelial cells, whereas 5-HTR4 was found predominantly in high-grade tumours. This result was confirmed by western blot analysis. At the mRNA level, 5-HTR4 mRNA was expressed in DU145 and LNCaP cells. Antagonists to both receptor subtypes inhibited proliferation of PC cells in a dose-dependent manner. Conclusions: The present result indicate that 5-HTRs are present at various tumour stages and that antagonists to these receptors can inhibit the proliferative activity of androgen-independent PC cell lines.

Original languageEnglish
Pages (from-to)895-900
Number of pages6
JournalEuropean Urology
Volume47
Issue number6
DOIs
Publication statusPublished - Jun 2005

Keywords

  • Androgen-independent tumour cells
  • BrdU assay
  • Immunohistochemistry
  • RT-PCR

ASJC Scopus subject areas

  • Urology

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