Expression of SpanX proteins in normal testes and in testicular germ cell tumours

Michele Salemi, Aldo E. Calogero, Roberto Castiglione, Dario Tricoli, Paola Asero, Romeo Rosa, Giancarlo Rappazzo, Enzo Vicari

Research output: Contribution to journalArticlepeer-review


We investigated the expression of the SpanX protein family in cells of normal testes and in testicular germ cell tumours, mainly seminomas and embryonal carcinomas, using an immunohistochemical approach. Most of the normal germ cells, belonging to spermatogonial and primary spermatocytic classes, showed a strong nuclear positivity. In contrast, post-meiotic germ cells showed diffused cytoplasmic and sometimes also perinuclear localization of the signal. The vast majority of cells were also positive in eight seminomas, six embryonal cell carcinomas and one teratocarcinoma. In all seminomas, nuclei were either exclusively or preferentially labelled; whereas, the nuclear signal intensity decreased in parallel with the appearance of some cytoplasmic staining in embryonal carcinomas. In conclusion, these data suggest that the SpanX protein family is not exclusively expressed post-meiotically and that seminomas and embryonal carcinomas may originate from SpanX-positive carcinoma-in-situ cell.

Original languageEnglish
Pages (from-to)368-373
Number of pages6
JournalInternational Journal of Andrology
Issue number2
Publication statusPublished - Apr 2006


  • Embryonal carcinoma
  • Immunohistochemistry
  • Seminoma
  • SpanX
  • Teratocarcinoma
  • Testicular tumour

ASJC Scopus subject areas

  • Endocrinology


Dive into the research topics of 'Expression of SpanX proteins in normal testes and in testicular germ cell tumours'. Together they form a unique fingerprint.

Cite this