Expression of stemness markers in mouse parthenogenetic-diploid blastocysts is influenced by slight variation of activation protocol adopted

Enrica Bianchi, Raffaele Geremia, Claudio Sette

Research output: Contribution to journalArticlepeer-review

Abstract

The importance of obtaining stem cells through alternative methods has increased progressively in the recent years due to the potential role that embryonic stem (ES) cells play in the field of regenerative medicine. In this regard, generation of parthenogenetic blastocysts allows the production of ethic-free ES cells without the need to manipulate normal embryos. Our work was aimed at clarifying whether variations in the method adopted to generate diploid parthenogenetic blastocysts could determine differences in the quality of blastocysts produced. In vitro development of mouse oocytes activated with three protocols, using Sr 2+ and cytochalasin for different time, was compared with that of in vivo fertilized embryos. We have evaluated the efficiency of blastocyst formation and analysed the expression pattern of the stemness markers OCT4, CDX2, and NANOG. Our results indicate that the yield of diploid parthenogenotes and the segregation of the stemness marker OCT4 in the developing blastocyst are influenced by the parthenogenetic protocol adopted. Particularly, even if all methods tested allowed the production of blastocysts in vitro, the correct segregation of OCT4 occurred only in blastocysts developed from oocytes concomitantly treated for 4 h with Sr 2+ and cytochalasin D. Our results indicate that the protocol employed to develop parthenogenetic blastocysts in vitro affects the quality of cells in the inner cell mass.

Original languageEnglish
Pages (from-to)619-623
Number of pages5
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume46
Issue number7
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Blastocysts
  • Cytochalasin D
  • Oct4
  • Parthenogenesis
  • Sr

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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