Abstract
Expression of the T cell receptor (TCR) genes is not restricted to T lymphocytes. Human prostate and breast express a truncated TCR gamma transcript. In the mouse, TCR alpha (TCRA) and beta partial transcripts are expressed by mesenchymal cells and TCRA transcripts by epithelial cells of the kidney. We show now that TCRA constant region expression is common in normal and neoplastic human cells of mesenchymal and neuroectodermal origin. TCR transcripts are derived from an unrearranged TCRA locus. Moreover, rhabdomyosarcoma cells highly expressed a specific J49-C splicing product deriving from the assembly of J49 segment and constant region. TCRA ectopic transcripts/proteins negatively regulate rhabdomyosarcoma cell growth as suggested by TCRA gene expression downmodulation effects using a specific duplex small interfering RNA.
| Original language | English |
|---|---|
| Pages (from-to) | 16-22 |
| Number of pages | 7 |
| Journal | Gene |
| Volume | 353 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jun 20 2005 |
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Keywords
- Ectopic expression
- Mesenchymal cells
ASJC Scopus subject areas
- Genetics
Cite this
Expression of T cell receptor alpha gene (TCRA) in human rhabdomyosarcoma and other musculo-skeletal sarcomas. / Croci, Stefania; Strippoli, Pierluigi; Bonsi, Laura; Bagnara, Gian Paolo; Guizzunti, Gianni; Sartini, Roberta; Tonelli, Roberto; Messina, Christian; Pierdomenico, Laura; Lollini, Pier Luigi.
In: Gene, Vol. 353, No. 1, 20.06.2005, p. 16-22.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of T cell receptor alpha gene (TCRA) in human rhabdomyosarcoma and other musculo-skeletal sarcomas
AU - Croci, Stefania
AU - Strippoli, Pierluigi
AU - Bonsi, Laura
AU - Bagnara, Gian Paolo
AU - Guizzunti, Gianni
AU - Sartini, Roberta
AU - Tonelli, Roberto
AU - Messina, Christian
AU - Pierdomenico, Laura
AU - Lollini, Pier Luigi
PY - 2005/6/20
Y1 - 2005/6/20
N2 - Expression of the T cell receptor (TCR) genes is not restricted to T lymphocytes. Human prostate and breast express a truncated TCR gamma transcript. In the mouse, TCR alpha (TCRA) and beta partial transcripts are expressed by mesenchymal cells and TCRA transcripts by epithelial cells of the kidney. We show now that TCRA constant region expression is common in normal and neoplastic human cells of mesenchymal and neuroectodermal origin. TCR transcripts are derived from an unrearranged TCRA locus. Moreover, rhabdomyosarcoma cells highly expressed a specific J49-C splicing product deriving from the assembly of J49 segment and constant region. TCRA ectopic transcripts/proteins negatively regulate rhabdomyosarcoma cell growth as suggested by TCRA gene expression downmodulation effects using a specific duplex small interfering RNA.
AB - Expression of the T cell receptor (TCR) genes is not restricted to T lymphocytes. Human prostate and breast express a truncated TCR gamma transcript. In the mouse, TCR alpha (TCRA) and beta partial transcripts are expressed by mesenchymal cells and TCRA transcripts by epithelial cells of the kidney. We show now that TCRA constant region expression is common in normal and neoplastic human cells of mesenchymal and neuroectodermal origin. TCR transcripts are derived from an unrearranged TCRA locus. Moreover, rhabdomyosarcoma cells highly expressed a specific J49-C splicing product deriving from the assembly of J49 segment and constant region. TCRA ectopic transcripts/proteins negatively regulate rhabdomyosarcoma cell growth as suggested by TCRA gene expression downmodulation effects using a specific duplex small interfering RNA.
KW - Ectopic expression
KW - Mesenchymal cells
UR - http://www.scopus.com/inward/record.url?scp=20444412615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20444412615&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2005.04.012
DO - 10.1016/j.gene.2005.04.012
M3 - Article
C2 - 15935573
AN - SCOPUS:20444412615
VL - 353
SP - 16
EP - 22
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -