Expression of tenascin is related to angiogenesis in pre-eclampsia

D. Ribatti, G. Loverro, A. Vacca, P. Greco, L. Roncali, L. Selvaggi

Research output: Contribution to journalArticle

Abstract

Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.

Original languageEnglish
Pages (from-to)373-378
Number of pages6
JournalEuropean Journal of Clinical Investigation
Volume28
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Tenascin
Decidua
Pre-Eclampsia
Microvessels
Chorioallantoic Membrane
Membranes
Angiogenesis Inducing Agents
Extracellular Matrix Proteins
Chick Embryo
Cesarean Section
Extracellular Matrix
Wheels
Tissue
Staining and Labeling
Growth

Keywords

  • Angiogenesis
  • Pre-eclampsia
  • Tenascin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ribatti, D., Loverro, G., Vacca, A., Greco, P., Roncali, L., & Selvaggi, L. (1998). Expression of tenascin is related to angiogenesis in pre-eclampsia. European Journal of Clinical Investigation, 28(5), 373-378. https://doi.org/10.1046/j.1365-2362.1998.00294.x

Expression of tenascin is related to angiogenesis in pre-eclampsia. / Ribatti, D.; Loverro, G.; Vacca, A.; Greco, P.; Roncali, L.; Selvaggi, L.

In: European Journal of Clinical Investigation, Vol. 28, No. 5, 1998, p. 373-378.

Research output: Contribution to journalArticle

Ribatti, D, Loverro, G, Vacca, A, Greco, P, Roncali, L & Selvaggi, L 1998, 'Expression of tenascin is related to angiogenesis in pre-eclampsia', European Journal of Clinical Investigation, vol. 28, no. 5, pp. 373-378. https://doi.org/10.1046/j.1365-2362.1998.00294.x
Ribatti, D. ; Loverro, G. ; Vacca, A. ; Greco, P. ; Roncali, L. ; Selvaggi, L. / Expression of tenascin is related to angiogenesis in pre-eclampsia. In: European Journal of Clinical Investigation. 1998 ; Vol. 28, No. 5. pp. 373-378.
@article{3ce96d325c1648ddb842a27b6e9c6fd9,
title = "Expression of tenascin is related to angiogenesis in pre-eclampsia",
abstract = "Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.",
keywords = "Angiogenesis, Pre-eclampsia, Tenascin",
author = "D. Ribatti and G. Loverro and A. Vacca and P. Greco and L. Roncali and L. Selvaggi",
year = "1998",
doi = "10.1046/j.1365-2362.1998.00294.x",
language = "English",
volume = "28",
pages = "373--378",
journal = "European Journal of Clinical Investigation",
issn = "0014-2972",
publisher = "Blackwell Publishing Ltd",
number = "5",

}

TY - JOUR

T1 - Expression of tenascin is related to angiogenesis in pre-eclampsia

AU - Ribatti, D.

AU - Loverro, G.

AU - Vacca, A.

AU - Greco, P.

AU - Roncali, L.

AU - Selvaggi, L.

PY - 1998

Y1 - 1998

N2 - Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.

AB - Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.

KW - Angiogenesis

KW - Pre-eclampsia

KW - Tenascin

UR - http://www.scopus.com/inward/record.url?scp=0031864302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031864302&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2362.1998.00294.x

DO - 10.1046/j.1365-2362.1998.00294.x

M3 - Article

C2 - 9650010

AN - SCOPUS:0031864302

VL - 28

SP - 373

EP - 378

JO - European Journal of Clinical Investigation

JF - European Journal of Clinical Investigation

SN - 0014-2972

IS - 5

ER -