Abstract
Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.
| Original language | English |
|---|---|
| Pages (from-to) | 373-378 |
| Number of pages | 6 |
| Journal | European Journal of Clinical Investigation |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1998 |
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Keywords
- Angiogenesis
- Pre-eclampsia
- Tenascin
ASJC Scopus subject areas
- Medicine(all)
Cite this
Expression of tenascin is related to angiogenesis in pre-eclampsia. / Ribatti, D.; Loverro, G.; Vacca, A.; Greco, P.; Roncali, L.; Selvaggi, L.
In: European Journal of Clinical Investigation, Vol. 28, No. 5, 1998, p. 373-378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of tenascin is related to angiogenesis in pre-eclampsia
AU - Ribatti, D.
AU - Loverro, G.
AU - Vacca, A.
AU - Greco, P.
AU - Roncali, L.
AU - Selvaggi, L.
PY - 1998
Y1 - 1998
N2 - Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.
AB - Methods: Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addition, pre-eclamptic and control samples were grafted onto the chick embryo chorioallantoic membrane (CAM) to study their possible angiogenic activity in vivo. Results: Although the microvessel area was low in control samples, it increased significantly in the pre-eclamptic decidua. In parallel, the area covered by tenascin staining underwent a significant expansion that was highly correlated with the microvessel area. Angiogenic activity was assessed both macroscopically and microscopically on hist, logical sections 4 days after grafting. In contrast to control samples, pre-eclamptic decidua induced an intense capillary growth, and numerous capillaries converging towards the implant and forming a spoke-wheel pattern were evident. Histological examination using a planimetric point-count method showed that microvessel counts in the CAM area under and around the pre- eclamptic implants were significantly higher than those of control samples. Again, the extracellular matrix of nearby microvessels was strongly immunoreactive with tenascin. Conclusion: It is suggested that, unlike normal decidua, pre-eclamptic decidua displays an intense, autonomous angiogenic activity, probably mediated by tenascin in addition to its own angiogenic factors.
KW - Angiogenesis
KW - Pre-eclampsia
KW - Tenascin
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UR - http://www.scopus.com/inward/citedby.url?scp=0031864302&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2362.1998.00294.x
DO - 10.1046/j.1365-2362.1998.00294.x
M3 - Article
C2 - 9650010
AN - SCOPUS:0031864302
VL - 28
SP - 373
EP - 378
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
SN - 0014-2972
IS - 5
ER -