Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS

Roberta Benfante, Ruth Adele Antonini, Maria De Pizzol, Cecilia Gotti, Francesco Clementi, Massimo Locati, Diego Fornasari

Research output: Contribution to journalArticle

Abstract

We investigated the expression of the partially duplicated α7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic α7 gene in these lineages, but real-time PCR showed the exclusive expression of α7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-κB as its specific inhibitor parthenolide prevented the reduction in the α7dup transcript. Such precise regulation suggests that α7dup may specifically participate in the inflammatory response of the innate immune system.

Original languageEnglish
Pages (from-to)74-84
Number of pages11
JournalJournal of Neuroimmunology
Volume230
Issue number1-2
DOIs
Publication statusPublished - Jan 2011

Keywords

  • CHRFAM7A
  • CHRNA7
  • LPS
  • Monocytes
  • NF-κB
  • Nicotinic acetylcholine receptor

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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