Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis

Donatella Granchi, Gabriela Ciapetti, Lucia Savarino, Susanna Stea, Federica Filippini, Alessandra Sudanese, Roberto Rotini, Armando Giunti

Research output: Contribution to journalArticlepeer-review


The aim of the study was to evaluate the sensitization to metals in patients with Co-Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced 'activation index' was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an 'activation index' significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as 'normal' (≥ 0.9 and

Original languageEnglish
Pages (from-to)2059-2065
Number of pages7
Issue number20
Publication statusPublished - Oct 2000


  • Activation antigen
  • Hip prosthesis
  • Lymphocyte sensitization
  • Metal corrosion

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


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