Abstract
The aim of the study was to evaluate the sensitization to metals in patients with Co-Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced 'activation index' was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an 'activation index' significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as 'normal' (≥ 0.9 and
| Original language | English |
|---|---|
| Pages (from-to) | 2059-2065 |
| Number of pages | 7 |
| Journal | Biomaterials |
| Volume | 21 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - Oct 2000 |
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Keywords
- Activation antigen
- Hip prosthesis
- Lymphocyte sensitization
- Metal corrosion
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis. / Granchi, Donatella; Ciapetti, Gabriela; Savarino, Lucia; Stea, Susanna; Filippini, Federica; Sudanese, Alessandra; Rotini, Roberto; Giunti, Armando.
In: Biomaterials, Vol. 21, No. 20, 10.2000, p. 2059-2065.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis
AU - Granchi, Donatella
AU - Ciapetti, Gabriela
AU - Savarino, Lucia
AU - Stea, Susanna
AU - Filippini, Federica
AU - Sudanese, Alessandra
AU - Rotini, Roberto
AU - Giunti, Armando
PY - 2000/10
Y1 - 2000/10
N2 - The aim of the study was to evaluate the sensitization to metals in patients with Co-Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced 'activation index' was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an 'activation index' significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as 'normal' (≥ 0.9 and
AB - The aim of the study was to evaluate the sensitization to metals in patients with Co-Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced 'activation index' was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an 'activation index' significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as 'normal' (≥ 0.9 and
KW - Activation antigen
KW - Hip prosthesis
KW - Lymphocyte sensitization
KW - Metal corrosion
UR - http://www.scopus.com/inward/record.url?scp=0034308365&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034308365&partnerID=8YFLogxK
U2 - 10.1016/S0142-9612(00)00099-5
DO - 10.1016/S0142-9612(00)00099-5
M3 - Article
C2 - 10966015
AN - SCOPUS:0034308365
VL - 21
SP - 2059
EP - 2065
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 20
ER -