Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: Relevance for natural killer-dendritic cell interaction

Daniela Pende, Roberta Castriconi, Paola Romagnani, Grazia Maria Spaggiari, Stefania Marcenaro, Alessandra Dondero, Elena Lazzeri, Laura Lasagni, Stefania Martini, Paola Rivera, Andrea Capobianco, Lorenzo Moretta, Alessandro Moretta, Cristina Bottino

Research output: Contribution to journalArticle

Abstract

In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.

Original languageEnglish
Pages (from-to)2030-2036
Number of pages7
JournalBlood
Volume107
Issue number5
DOIs
Publication statusPublished - Mar 1 2006

ASJC Scopus subject areas

  • Hematology

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    Pende, D., Castriconi, R., Romagnani, P., Spaggiari, G. M., Marcenaro, S., Dondero, A., Lazzeri, E., Lasagni, L., Martini, S., Rivera, P., Capobianco, A., Moretta, L., Moretta, A., & Bottino, C. (2006). Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: Relevance for natural killer-dendritic cell interaction. Blood, 107(5), 2030-2036. https://doi.org/10.1182/blood-2005-07-2696