Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: Relevance for natural killer-dendritic cell interaction

Daniela Pende, Roberta Castriconi, Paola Romagnani, Grazia Maria Spaggiari, Stefania Marcenaro, Alessandra Dondero, Elena Lazzeri, Laura Lasagni, Stefania Martini, Paola Rivera, Andrea Capobianco, Lorenzo Moretta, Alessandro Moretta, Cristina Bottino

Research output: Contribution to journalArticle

Abstract

In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.

Original languageEnglish
Pages (from-to)2030-2036
Number of pages7
JournalBlood
Volume107
Issue number5
DOIs
Publication statusPublished - Mar 1 2006

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Cell Communication
Natural Killer Cells
Dendritic Cells
Ligands
Flagellin
Poly I-C
CD40 Ligand
T-cells
Venules
Confocal microscopy
Confocal Microscopy
Lipopolysaccharides
Monocytes
Assays
Lymph Nodes
poliovirus receptor
nectins
T-Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells : Relevance for natural killer-dendritic cell interaction. / Pende, Daniela; Castriconi, Roberta; Romagnani, Paola; Spaggiari, Grazia Maria; Marcenaro, Stefania; Dondero, Alessandra; Lazzeri, Elena; Lasagni, Laura; Martini, Stefania; Rivera, Paola; Capobianco, Andrea; Moretta, Lorenzo; Moretta, Alessandro; Bottino, Cristina.

In: Blood, Vol. 107, No. 5, 01.03.2006, p. 2030-2036.

Research output: Contribution to journalArticle

Pende, D, Castriconi, R, Romagnani, P, Spaggiari, GM, Marcenaro, S, Dondero, A, Lazzeri, E, Lasagni, L, Martini, S, Rivera, P, Capobianco, A, Moretta, L, Moretta, A & Bottino, C 2006, 'Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: Relevance for natural killer-dendritic cell interaction', Blood, vol. 107, no. 5, pp. 2030-2036. https://doi.org/10.1182/blood-2005-07-2696
Pende, Daniela ; Castriconi, Roberta ; Romagnani, Paola ; Spaggiari, Grazia Maria ; Marcenaro, Stefania ; Dondero, Alessandra ; Lazzeri, Elena ; Lasagni, Laura ; Martini, Stefania ; Rivera, Paola ; Capobianco, Andrea ; Moretta, Lorenzo ; Moretta, Alessandro ; Bottino, Cristina. / Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells : Relevance for natural killer-dendritic cell interaction. In: Blood. 2006 ; Vol. 107, No. 5. pp. 2030-2036.
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AU - Lazzeri, Elena

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AU - Rivera, Paola

AU - Capobianco, Andrea

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