TY - JOUR
T1 - Expression of the epidermal growth factor receptor gene in human intestinal metaplasia
T2 - A preliminary report
AU - Granelli, P.
AU - Fichera, G.
AU - Zennaro, F.
AU - Siardi, C.
AU - De Ruberto, F.
AU - Fregoni, F.
AU - Appierto, V.
AU - Buffa, R.
AU - Ferrero, S.
AU - Biunno, I.
PY - 1997
Y1 - 1997
N2 - Background: The role of growth factors/receptors in the etiopathology and/or development of gastric cancer has recently come under scrutiny, since overexpression or amplification of the EGF system has been found in many intestinal type gastric cancers and related to a more aggressive behavior. Since these gastric carcinomas appear to develop from intestinal metaplasia, a study was planned to investigate whether overexpression of the EGF- receptor gene also occurred in intestinal metaplastic mucosa. Methods: Patients underwent upper GL endoscopy. Gastric biopsies for routine histology, Helicobacter pylori detection, quantification of intestinal metaplasia and EGF-R expression analysis were performed. A 30mer EGF-R specific oligonucleotide was end-labeled and used to probe a dot blot filter containing the RNA from the bioptic samples. Results: Though all the gastric samples transcribed the EGF-R gene to a detectable level, overexpression of the EGF-R gene was found in the metaplastic mucosa in a minority of patients. Conclusions: These preliminary findings suggest that overexpression of the EGF-R gene is infrequent in the metaplastic gastric mucosa.
AB - Background: The role of growth factors/receptors in the etiopathology and/or development of gastric cancer has recently come under scrutiny, since overexpression or amplification of the EGF system has been found in many intestinal type gastric cancers and related to a more aggressive behavior. Since these gastric carcinomas appear to develop from intestinal metaplasia, a study was planned to investigate whether overexpression of the EGF- receptor gene also occurred in intestinal metaplastic mucosa. Methods: Patients underwent upper GL endoscopy. Gastric biopsies for routine histology, Helicobacter pylori detection, quantification of intestinal metaplasia and EGF-R expression analysis were performed. A 30mer EGF-R specific oligonucleotide was end-labeled and used to probe a dot blot filter containing the RNA from the bioptic samples. Results: Though all the gastric samples transcribed the EGF-R gene to a detectable level, overexpression of the EGF-R gene was found in the metaplastic mucosa in a minority of patients. Conclusions: These preliminary findings suggest that overexpression of the EGF-R gene is infrequent in the metaplastic gastric mucosa.
KW - Epidermal growth factor receptor
KW - intestinal metaplasia
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M3 - Article
C2 - 9175212
AN - SCOPUS:8244261279
VL - 32
SP - 485
EP - 489
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 5
ER -