Expression of the interleukin-2 on human fibroblasts and its biological significance

S. Plaisance, E. Rubinstein, A. Alileche, Y. Sahraoui, P. Krief, Y. Augery-Bourget, C. Jasmin, H. Suarez, B. Azzarone

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Abstract

In this study, we have investigated the expression of the α and β chains of the IL-2 receptor (IL-2Rα, IL-2Rβ) both at the membrane and at transcriptional levels during the lifespan of human embryonic fibroblasts. Here we show that the mAbs IOT14 and MIKβ1 directed against the IL-2 binding sites of the IL-2Rα and IL-2Rβ respectively, stain human embryonic fibroblasts early in their life span. Data from [ 125I]rIL2 cross-linking experiments show the simultaneous expression of two IL-2 binding peptides of 70 and 55 kDa respectively on embryonic young fibroblasts as on lymphoid activated cells. The p55 and the p70 IL-2 binding peptides are shown to be specific for the IL-2Rα and to the IL-2Rβ by the finding that these bands are abolished by excess amounts of cold IL-2 and mAbs directed against the IL-2 binding sites of the α and β chains. Scatchard analysis after [ 125I]IL-2 labelling shows the presence of both high affinity (150 sites with a K(d) of 147 pM) and low affinity (1100 sites with a K(d) of 4 nM) IL-2 binding sites. Northern blot and dot blot analysis show the presence of specific transcripts for the IL-2Rα and IL-2Rβ genes in early passaged fibroblasts. By contrast, in senescent cultures, only the IL-2Rβ transcript were detected. Finally, IL-2 at low concentrations (36 pM) down modulates the level of the intercellular adhesion molecule ICAM-1 in young but not in senescent cultures. Changes in the expression of the IL-2Rα during aging process probably affect the regulation of the ICAM-1 dependent fibroblastic functions.

Original languageEnglish
Pages (from-to)739-746
Number of pages8
JournalInternational Immunology
Volume4
Issue number7
Publication statusPublished - 1992

Fingerprint

Interleukin
Fibroblasts
Interleukin-2
Binding sites
Peptides
Life Span
p.m.
Intercellular Adhesion Molecule-1
Affine transformation
Adhesion Molecules
Binding Sites
Cell culture
Labeling
Adhesion
Genes
Aging of materials
Receptor
Linking
Excess
Membranes

Keywords

  • aging
  • fibroblasts
  • ICAM-1
  • IL-2
  • IL-2R
  • IL-2Rα
  • IL-2Rβ

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Immunology
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Transplantation
  • Neuropsychology and Physiological Psychology

Cite this

Plaisance, S., Rubinstein, E., Alileche, A., Sahraoui, Y., Krief, P., Augery-Bourget, Y., ... Azzarone, B. (1992). Expression of the interleukin-2 on human fibroblasts and its biological significance. International Immunology, 4(7), 739-746.

Expression of the interleukin-2 on human fibroblasts and its biological significance. / Plaisance, S.; Rubinstein, E.; Alileche, A.; Sahraoui, Y.; Krief, P.; Augery-Bourget, Y.; Jasmin, C.; Suarez, H.; Azzarone, B.

In: International Immunology, Vol. 4, No. 7, 1992, p. 739-746.

Research output: Contribution to journalArticle

Plaisance, S, Rubinstein, E, Alileche, A, Sahraoui, Y, Krief, P, Augery-Bourget, Y, Jasmin, C, Suarez, H & Azzarone, B 1992, 'Expression of the interleukin-2 on human fibroblasts and its biological significance', International Immunology, vol. 4, no. 7, pp. 739-746.
Plaisance S, Rubinstein E, Alileche A, Sahraoui Y, Krief P, Augery-Bourget Y et al. Expression of the interleukin-2 on human fibroblasts and its biological significance. International Immunology. 1992;4(7):739-746.
Plaisance, S. ; Rubinstein, E. ; Alileche, A. ; Sahraoui, Y. ; Krief, P. ; Augery-Bourget, Y. ; Jasmin, C. ; Suarez, H. ; Azzarone, B. / Expression of the interleukin-2 on human fibroblasts and its biological significance. In: International Immunology. 1992 ; Vol. 4, No. 7. pp. 739-746.
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