Expression of the Met/HGF receptor in normal and neoplastic human tissues

M. F. Di Renzo, R. P. Narsimhan, M. Olivero, S. Bretti, S. Giordano, E. Medico, P. Gaglia, P. Zara, P. M. Comoglio

Research output: Contribution to journalArticle

Abstract

The MET oncogene encodes a transmembrane tyrosine kinase receptor. Recently, hepatocyte growth factor (HGF), a potent growth factor for hepatocytes involved in liver regeneration, has been proposed as a ligand. In this paper, the physiological role of the human Met/HGF receptor is investigated by studying its specific distribution in normal and neoplastic tissues. Northern blot analysis has shown that the MET gene is selectively expressed in several epithelial tissues. High levels of MET mRNA have been found in liver, gastrointestinal tract, thyroid and kidney. Western blot analysis has shown that the levels of the Met protein generally correspond to those of the mRNA. However, in the thyroid, where there is a high level of MET mRNA, the protein was barely detectable, suggesting translational or posttranslational regulation. The protein was also detected in the brain. Normal or increased levels of MET mRNA and Met protein were consistently found in fresh samples of carcinomas as well as in epithelial tumor cell lines. In thyroid carcinomas of a. specific histiotype the amount of Met protein, almost undetectable in the normal counterpart, was found to be increased more than 100-fold. The tissue distribution of the Met/HGF receptor indicates that this molecule is involved in growth control of epithelial cells other than hepatocytes and suggests that its increased expression may confer a growth advantage to neoplastic cells.

Original languageEnglish
Pages (from-to)1997-2003
Number of pages7
JournalOncogene
Volume6
Issue number11
Publication statusPublished - Nov 1991

Fingerprint

Proto-Oncogene Proteins c-met
Messenger RNA
Hepatocyte Growth Factor
Proteins
Thyroid Gland
Epithelial Cells
Liver Regeneration
Normal Distribution
Receptor Protein-Tyrosine Kinases
Tissue Distribution
Growth
Tumor Cell Line
Oncogenes
Thyroid Neoplasms
Northern Blotting
Gastrointestinal Tract
Hepatocytes
Epithelium
Western Blotting
Ligands

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Expression of the Met/HGF receptor in normal and neoplastic human tissues. / Di Renzo, M. F.; Narsimhan, R. P.; Olivero, M.; Bretti, S.; Giordano, S.; Medico, E.; Gaglia, P.; Zara, P.; Comoglio, P. M.

In: Oncogene, Vol. 6, No. 11, 11.1991, p. 1997-2003.

Research output: Contribution to journalArticle

Di Renzo, MF, Narsimhan, RP, Olivero, M, Bretti, S, Giordano, S, Medico, E, Gaglia, P, Zara, P & Comoglio, PM 1991, 'Expression of the Met/HGF receptor in normal and neoplastic human tissues', Oncogene, vol. 6, no. 11, pp. 1997-2003.
Di Renzo, M. F. ; Narsimhan, R. P. ; Olivero, M. ; Bretti, S. ; Giordano, S. ; Medico, E. ; Gaglia, P. ; Zara, P. ; Comoglio, P. M. / Expression of the Met/HGF receptor in normal and neoplastic human tissues. In: Oncogene. 1991 ; Vol. 6, No. 11. pp. 1997-2003.
@article{ea94812ac641485ca9f7116d57b33f90,
title = "Expression of the Met/HGF receptor in normal and neoplastic human tissues",
abstract = "The MET oncogene encodes a transmembrane tyrosine kinase receptor. Recently, hepatocyte growth factor (HGF), a potent growth factor for hepatocytes involved in liver regeneration, has been proposed as a ligand. In this paper, the physiological role of the human Met/HGF receptor is investigated by studying its specific distribution in normal and neoplastic tissues. Northern blot analysis has shown that the MET gene is selectively expressed in several epithelial tissues. High levels of MET mRNA have been found in liver, gastrointestinal tract, thyroid and kidney. Western blot analysis has shown that the levels of the Met protein generally correspond to those of the mRNA. However, in the thyroid, where there is a high level of MET mRNA, the protein was barely detectable, suggesting translational or posttranslational regulation. The protein was also detected in the brain. Normal or increased levels of MET mRNA and Met protein were consistently found in fresh samples of carcinomas as well as in epithelial tumor cell lines. In thyroid carcinomas of a. specific histiotype the amount of Met protein, almost undetectable in the normal counterpart, was found to be increased more than 100-fold. The tissue distribution of the Met/HGF receptor indicates that this molecule is involved in growth control of epithelial cells other than hepatocytes and suggests that its increased expression may confer a growth advantage to neoplastic cells.",
author = "{Di Renzo}, {M. F.} and Narsimhan, {R. P.} and M. Olivero and S. Bretti and S. Giordano and E. Medico and P. Gaglia and P. Zara and Comoglio, {P. M.}",
year = "1991",
month = "11",
language = "English",
volume = "6",
pages = "1997--2003",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - Expression of the Met/HGF receptor in normal and neoplastic human tissues

AU - Di Renzo, M. F.

AU - Narsimhan, R. P.

AU - Olivero, M.

AU - Bretti, S.

AU - Giordano, S.

AU - Medico, E.

AU - Gaglia, P.

AU - Zara, P.

AU - Comoglio, P. M.

PY - 1991/11

Y1 - 1991/11

N2 - The MET oncogene encodes a transmembrane tyrosine kinase receptor. Recently, hepatocyte growth factor (HGF), a potent growth factor for hepatocytes involved in liver regeneration, has been proposed as a ligand. In this paper, the physiological role of the human Met/HGF receptor is investigated by studying its specific distribution in normal and neoplastic tissues. Northern blot analysis has shown that the MET gene is selectively expressed in several epithelial tissues. High levels of MET mRNA have been found in liver, gastrointestinal tract, thyroid and kidney. Western blot analysis has shown that the levels of the Met protein generally correspond to those of the mRNA. However, in the thyroid, where there is a high level of MET mRNA, the protein was barely detectable, suggesting translational or posttranslational regulation. The protein was also detected in the brain. Normal or increased levels of MET mRNA and Met protein were consistently found in fresh samples of carcinomas as well as in epithelial tumor cell lines. In thyroid carcinomas of a. specific histiotype the amount of Met protein, almost undetectable in the normal counterpart, was found to be increased more than 100-fold. The tissue distribution of the Met/HGF receptor indicates that this molecule is involved in growth control of epithelial cells other than hepatocytes and suggests that its increased expression may confer a growth advantage to neoplastic cells.

AB - The MET oncogene encodes a transmembrane tyrosine kinase receptor. Recently, hepatocyte growth factor (HGF), a potent growth factor for hepatocytes involved in liver regeneration, has been proposed as a ligand. In this paper, the physiological role of the human Met/HGF receptor is investigated by studying its specific distribution in normal and neoplastic tissues. Northern blot analysis has shown that the MET gene is selectively expressed in several epithelial tissues. High levels of MET mRNA have been found in liver, gastrointestinal tract, thyroid and kidney. Western blot analysis has shown that the levels of the Met protein generally correspond to those of the mRNA. However, in the thyroid, where there is a high level of MET mRNA, the protein was barely detectable, suggesting translational or posttranslational regulation. The protein was also detected in the brain. Normal or increased levels of MET mRNA and Met protein were consistently found in fresh samples of carcinomas as well as in epithelial tumor cell lines. In thyroid carcinomas of a. specific histiotype the amount of Met protein, almost undetectable in the normal counterpart, was found to be increased more than 100-fold. The tissue distribution of the Met/HGF receptor indicates that this molecule is involved in growth control of epithelial cells other than hepatocytes and suggests that its increased expression may confer a growth advantage to neoplastic cells.

UR - http://www.scopus.com/inward/record.url?scp=0025999940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025999940&partnerID=8YFLogxK

M3 - Article

C2 - 1719465

AN - SCOPUS:0025999940

VL - 6

SP - 1997

EP - 2003

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 11

ER -