Expression of the Rai (Shc C) adaptor protein in the human enteric nervous system

V. Villanacci, G. Bassotti, B. Ortensi, S. Fisogni, G. Cathomas, C. A. Maurer, A. Galletti, B. Salerni, G. Pelicci

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The adaptor protein Rai (ShcC/N-Shc) is almost exclusively present in the nervous system, although little is documented about its expression in the gut and the enteric nervous system (ENS). As Rai is a physiological substrate of Ret, an important factor for the development of ENS, we have evaluated the expression of Rai in the ENS in various segments of the human gastrointestinal tract. The expression of Rai was assessed by immunohistochemistry in disease-free human gut samples (oesophagus, stomach, small bowel and colon) obtained from subjects undergoing surgical procedures. Rai was not expressed in the epithelia or lymphoid tissue, whereas a moderate level of expression was observed in the endothelial cells of blood vessels and on the outer membrane of smooth muscle cells in both the muscularis mucosae and the muscularis propria. In the ENS, strong positivity was observed only in enteric glial cells, overlapping with GFAP and S100. In conclusion, Rai is expressed in the human gut, especially in the enteric glial cells. We conclude that Rai may provide an additional marker for this cell type.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalNeurogastroenterology and Motility
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Shc Signaling Adaptor Proteins
Enteric Nervous System
Neuroglia
Lymphoid Tissue
Nervous System
Esophagus
Smooth Muscle Myocytes
Blood Vessels
Gastrointestinal Tract
Stomach
Colon
Mucous Membrane
Epithelium
Endothelial Cells
Immunohistochemistry
Membranes
Proteins

Keywords

  • Enteric glial cells
  • Enteric neurons
  • Gastrointestinal tract
  • Rai

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this

Expression of the Rai (Shc C) adaptor protein in the human enteric nervous system. / Villanacci, V.; Bassotti, G.; Ortensi, B.; Fisogni, S.; Cathomas, G.; Maurer, C. A.; Galletti, A.; Salerni, B.; Pelicci, G.

In: Neurogastroenterology and Motility, Vol. 20, No. 3, 03.2008, p. 206-212.

Research output: Contribution to journalArticle

Villanacci, V, Bassotti, G, Ortensi, B, Fisogni, S, Cathomas, G, Maurer, CA, Galletti, A, Salerni, B & Pelicci, G 2008, 'Expression of the Rai (Shc C) adaptor protein in the human enteric nervous system', Neurogastroenterology and Motility, vol. 20, no. 3, pp. 206-212. https://doi.org/10.1111/j.1365-2982.2007.01017.x
Villanacci, V. ; Bassotti, G. ; Ortensi, B. ; Fisogni, S. ; Cathomas, G. ; Maurer, C. A. ; Galletti, A. ; Salerni, B. ; Pelicci, G. / Expression of the Rai (Shc C) adaptor protein in the human enteric nervous system. In: Neurogastroenterology and Motility. 2008 ; Vol. 20, No. 3. pp. 206-212.
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