Expression of the Xist gene in urogenital ridges of midgestation male embryos

S. Dolci, R. Geremia, C. Albanesi, P. Rossi

Research output: Contribution to journalArticle

Abstract

We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.

Original languageEnglish
Pages (from-to)334-340
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume205
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Embryonic Structures
Genes
Cells
Chromosomes
Gene Expression
Germ Cells
Testis
X Chromosome Inactivation
Spermatids
Haploidy
Gonads
X Chromosome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of the Xist gene in urogenital ridges of midgestation male embryos. / Dolci, S.; Geremia, R.; Albanesi, C.; Rossi, P.

In: Biochemical and Biophysical Research Communications, Vol. 205, No. 1, 1994, p. 334-340.

Research output: Contribution to journalArticle

@article{d2e3373294e34e219b7c22bb67512668,
title = "Expression of the Xist gene in urogenital ridges of midgestation male embryos",
abstract = "We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.",
author = "S. Dolci and R. Geremia and C. Albanesi and P. Rossi",
year = "1994",
doi = "10.1006/bbrc.1994.2669",
language = "English",
volume = "205",
pages = "334--340",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Expression of the Xist gene in urogenital ridges of midgestation male embryos

AU - Dolci, S.

AU - Geremia, R.

AU - Albanesi, C.

AU - Rossi, P.

PY - 1994

Y1 - 1994

N2 - We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.

AB - We show by RT-PCR analysis that transcripts from the Xist gene, which is normally expressed from the inactive X chromosome of female somatic cells and postnatal male germ cells, are transiently expressed also in male embryos around the time of testis differentiation, mainly in somatic cells of urogenital ridges. In the postnatal testis, we find that Xist transcripts are mainly localized within the nucleus of haploid spermatids. These findings suggest that inactivation of the X-chromosome might occur not only in adult male germ cells, but also, transiently, in somatic cells of the male urogenital ridge. Both in the embryonal gonad and in differentiating germ cells Xist expression in males overlaps the pattern of expression of the testis determining gene Sry.

UR - http://www.scopus.com/inward/record.url?scp=0028102662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028102662&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1994.2669

DO - 10.1006/bbrc.1994.2669

M3 - Article

C2 - 7999045

AN - SCOPUS:0028102662

VL - 205

SP - 334

EP - 340

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -