Expression of toll-like receptors in healthy and allergic conjunctiva

Stefano Bonini, Alessandra Micera, Alfonso Iovieno, Alessandro Lambiase, Sergio Bonini

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.

Original languageEnglish
JournalOphthalmology
Volume112
Issue number9
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Allergic Conjunctivitis
Toll-Like Receptors
Conjunctiva
Toll-Like Receptor 9
Toll-Like Receptor 4
Toll-Like Receptor 2
Eosinophils
Mast Cells
Down-Regulation
Lymphocytes
Confocal Microscopy
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Up-Regulation
Epithelium
Immunohistochemistry
Outcome Assessment (Health Care)
T-Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Expression of toll-like receptors in healthy and allergic conjunctiva. / Bonini, Stefano; Micera, Alessandra; Iovieno, Alfonso; Lambiase, Alessandro; Bonini, Sergio.

In: Ophthalmology, Vol. 112, No. 9, 09.2005.

Research output: Contribution to journalArticle

Bonini, Stefano ; Micera, Alessandra ; Iovieno, Alfonso ; Lambiase, Alessandro ; Bonini, Sergio. / Expression of toll-like receptors in healthy and allergic conjunctiva. In: Ophthalmology. 2005 ; Vol. 112, No. 9.
@article{57d453070c6b4692918645266ee7427d,
title = "Expression of toll-like receptors in healthy and allergic conjunctiva",
abstract = "Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.",
author = "Stefano Bonini and Alessandra Micera and Alfonso Iovieno and Alessandro Lambiase and Sergio Bonini",
year = "2005",
month = "9",
doi = "10.1016/j.ophtha.2005.04.009",
language = "English",
volume = "112",
journal = "Ophthalmology",
issn = "0161-6420",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Expression of toll-like receptors in healthy and allergic conjunctiva

AU - Bonini, Stefano

AU - Micera, Alessandra

AU - Iovieno, Alfonso

AU - Lambiase, Alessandro

AU - Bonini, Sergio

PY - 2005/9

Y1 - 2005/9

N2 - Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.

AB - Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.

UR - http://www.scopus.com/inward/record.url?scp=24944480858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944480858&partnerID=8YFLogxK

U2 - 10.1016/j.ophtha.2005.04.009

DO - 10.1016/j.ophtha.2005.04.009

M3 - Article

C2 - 16023216

AN - SCOPUS:24944480858

VL - 112

JO - Ophthalmology

JF - Ophthalmology

SN - 0161-6420

IS - 9

ER -