Abstract
Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.
| Original language | English |
|---|---|
| Journal | Ophthalmology |
| Volume | 112 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2005 |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Expression of toll-like receptors in healthy and allergic conjunctiva. / Bonini, Stefano; Micera, Alessandra; Iovieno, Alfonso; Lambiase, Alessandro; Bonini, Sergio.
In: Ophthalmology, Vol. 112, No. 9, 09.2005.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of toll-like receptors in healthy and allergic conjunctiva
AU - Bonini, Stefano
AU - Micera, Alessandra
AU - Iovieno, Alfonso
AU - Lambiase, Alessandro
AU - Bonini, Sergio
PY - 2005/9
Y1 - 2005/9
N2 - Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.
AB - Purpose: To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. Design: Comparative nonrandomized interventional study. Participants: Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. Methods: Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and TLR-9 mRNA and proteins by relative real-time polymerase chain reaction and immunohistochemistry. Colocalization of TLRs was further evaluated by confocal microscopy and double-labeled immunofluorescence in T cells (CD4), mast cells (AA1), and eosinophils (EG2). Main Outcome Measures: Identification of TLRs at molecular and biochemical levels and identification of inflammatory cells that coexpress these receptors. Results: Toll-like receptor 2, TLR-4, and TLR-9 were expressed in normal and VKC conjunctival epithelium and stroma, both with a more intense immunoreactivity in the stroma. In VKC tissues, TLR-4 was upregulated, TLR-9 was downregulated, and TLR-2 was slightly decreased relative to normal tissues. Toll-like receptor 4 was localized on CD4+ lymphocytes, eosinophils, and mast cells, whereas lymphocytes and eosinophils, but not mast cells, expressed TLR-9. Conclusions: Toll-like receptors are expressed in healthy and allergic conjunctiva. The upregulation or downregulation of these receptors and their presence on inflammatory cells suggest a role of TLRs in VKC.
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UR - http://www.scopus.com/inward/citedby.url?scp=24944480858&partnerID=8YFLogxK
U2 - 10.1016/j.ophtha.2005.04.009
DO - 10.1016/j.ophtha.2005.04.009
M3 - Article
C2 - 16023216
AN - SCOPUS:24944480858
VL - 112
JO - Ophthalmology
JF - Ophthalmology
SN - 0161-6420
IS - 9
ER -