Expression of TP53 mutation-associated microRNAs predicts clinical outcome in head and neck squamous cell carcinoma patients

F. Ganci, A. Sacconi, N. Bossel Ben-Moshe, V. Manciocco, I. Sperduti, L. Strigari, R. Covello, M. Benevolo, E. Pescarmona, E. Domany, P. Muti, S. Strano, G. Spriano, G. Fontemaggi, Giovanni Blandino

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background: TP53 mutation is associated with decreased survival rate in head and neck squamous cell carcinoma (HNSCC) patients. We set out to identify microRNAs (miRNAs) whose expression associates with TP53 mutation and survival in HNSCC. Patients and methods: We analyzed TP53 status by direct sequencing of exons 2 through 11 of a prospective series of 121 HNSCC samples and assessed its association with outcome in 109 followed-up patients. We carried out miRNA expression profiling on 121 HNSCC samples and 66 normal counterparts. miRNA associations with TP53 mutations and outcome were evaluated. Results: A TP53 mutation was present in 58% of the tumors and TP53 mutations were significantly associated with a shorter recurrence-free survival. This association was stronger in the clinical subgroup of patients subjected to adjuvant therapy after surgery. The expression of 49 miRNAs was significantly associated with TP53 status. Among these 49, we identified a group of 12 miRNAs whose expression correlates with recurrence-free survival and a group of 4 miRNAs that correlates with cancer-specific survival. The two groups share three miRNAs. Importantly, miRNAs that correlate with survival are independent prognostic factors either when considered individually or as signatures. Conclusions: miRNAs expression associates with TP53 status and with reduced survival after surgical treatment of squamous cell carcinoma of the head and neck.

Original languageEnglish
Article numbermdt380
Pages (from-to)3082-3088
Number of pages7
JournalAnnals of Oncology
Volume24
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

MicroRNAs
Mutation
Survival
Carcinoma, squamous cell of head and neck
Recurrence
Exons
Neoplasms
Survival Rate
Therapeutics

Keywords

  • Clinical outcome
  • HNSCC
  • MicroRNA
  • TP53 mutation

ASJC Scopus subject areas

  • Oncology
  • Hematology

Cite this

Expression of TP53 mutation-associated microRNAs predicts clinical outcome in head and neck squamous cell carcinoma patients. / Ganci, F.; Sacconi, A.; Bossel Ben-Moshe, N.; Manciocco, V.; Sperduti, I.; Strigari, L.; Covello, R.; Benevolo, M.; Pescarmona, E.; Domany, E.; Muti, P.; Strano, S.; Spriano, G.; Fontemaggi, G.; Blandino, Giovanni.

In: Annals of Oncology, Vol. 24, No. 12, mdt380, 12.2013, p. 3082-3088.

Research output: Contribution to journalArticle

Ganci, F, Sacconi, A, Bossel Ben-Moshe, N, Manciocco, V, Sperduti, I, Strigari, L, Covello, R, Benevolo, M, Pescarmona, E, Domany, E, Muti, P, Strano, S, Spriano, G, Fontemaggi, G & Blandino, G 2013, 'Expression of TP53 mutation-associated microRNAs predicts clinical outcome in head and neck squamous cell carcinoma patients', Annals of Oncology, vol. 24, no. 12, mdt380, pp. 3082-3088. https://doi.org/10.1093/annonc/mdt380
Ganci, F. ; Sacconi, A. ; Bossel Ben-Moshe, N. ; Manciocco, V. ; Sperduti, I. ; Strigari, L. ; Covello, R. ; Benevolo, M. ; Pescarmona, E. ; Domany, E. ; Muti, P. ; Strano, S. ; Spriano, G. ; Fontemaggi, G. ; Blandino, Giovanni. / Expression of TP53 mutation-associated microRNAs predicts clinical outcome in head and neck squamous cell carcinoma patients. In: Annals of Oncology. 2013 ; Vol. 24, No. 12. pp. 3082-3088.
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AU - Sperduti, I.

AU - Strigari, L.

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AU - Domany, E.

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AU - Strano, S.

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