Expression of transcription factor Yin Yang 1 in human osteosarcomas

Filomena de Nigris, Chiara Botti, Anna de Chiara, Raffaele Rossiello, Gaetano Apice, Flavio Fazioli, Carmela Fiorito, Vincenzo Sica, Claudio Napoli

Research output: Contribution to journalArticle

Abstract

The transcription factor Yin Yang 1 (YY1) is known to be present in some human cancer cell lines and its expression correlates with immune-mediated apoptosis. By using Western blot analysis, we have shown that the YY1 protein is strongly expressed in human osteosarcoma cells and localised mainly in the nucleus. Moreover, by using immunohistochemistry and RT-PCR techniques, we have analysed the expression of YY1 protein in biopsies from human osteosarcomas. The YY1 protein was not detectable by immunohistochemistry in osteoid tissue. However, its expression was restricted to osteosarcoma tissues. These data were confirmed by densitometric analysis of RT-PCR for YY1 expression. Thus, YY1 gene activation appears to be an early event in the process of osteoblastic transformation and its detection may represent, together with the analysis of other established markers, a useful diagnostic tool in human osteosarcomas.

Original languageEnglish
Pages (from-to)2420-2424
Number of pages5
JournalEuropean Journal of Cancer
Volume42
Issue number15
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Osteosarcoma
  • Transcription factor
  • YY1

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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