Expression of transformation markers and suppression of tumorigenicity in human cell hybrids

L. Larizza, M. L. Tenchini, A. Mottura, L. De Carli, M. Colombi, S. Barlati

Research output: Contribution to journalArticle

Abstract

Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalEuropean Journal of Cancer and Clinical Oncology
Volume18
Issue number9
DOIs
Publication statusPublished - 1982

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Hybrid Cells
Plasminogen Activators
HeLa Cells
Fibronectins
Organism Cloning
Diploidy
Nude Mice
Agar
Neoplasms
Fibroblasts
Cell Line

ASJC Scopus subject areas

  • Oncology

Cite this

Expression of transformation markers and suppression of tumorigenicity in human cell hybrids. / Larizza, L.; Tenchini, M. L.; Mottura, A.; De Carli, L.; Colombi, M.; Barlati, S.

In: European Journal of Cancer and Clinical Oncology, Vol. 18, No. 9, 1982, p. 845-851.

Research output: Contribution to journalArticle

Larizza, L. ; Tenchini, M. L. ; Mottura, A. ; De Carli, L. ; Colombi, M. ; Barlati, S. / Expression of transformation markers and suppression of tumorigenicity in human cell hybrids. In: European Journal of Cancer and Clinical Oncology. 1982 ; Vol. 18, No. 9. pp. 845-851.
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