Abstract
Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.
| Original language | English |
|---|---|
| Pages (from-to) | 845-851 |
| Number of pages | 7 |
| Journal | European Journal of Cancer and Clinical Oncology |
| Volume | 18 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1982 |
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ASJC Scopus subject areas
- Oncology
Cite this
Expression of transformation markers and suppression of tumorigenicity in human cell hybrids. / Larizza, L.; Tenchini, M. L.; Mottura, A.; De Carli, L.; Colombi, M.; Barlati, S.
In: European Journal of Cancer and Clinical Oncology, Vol. 18, No. 9, 1982, p. 845-851.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of transformation markers and suppression of tumorigenicity in human cell hybrids
AU - Larizza, L.
AU - Tenchini, M. L.
AU - Mottura, A.
AU - De Carli, L.
AU - Colombi, M.
AU - Barlati, S.
PY - 1982
Y1 - 1982
N2 - Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.
AB - Somatic human cell hybrids produced by fusion of HeLa cells and diploid fibroblasts were analysed in a study designed to test the coordinate expression of transformation markers and tumorigenicity. The great majority of these hybrids displayed a finite lifespan in culture, but some of them inherited from the HeLa parent the capability to grow as permanent cell lines. Hybrids from both groups all had a plasminogen activator activity 20 to 100-fold higher and a cloning efficiency in semisolid medium 2 to 10-fold lower than the HeLa parent. Cell surface fibronectin was expressed at variable levels, albeit in a disorganized form. No correlation between the level of plasminogen activator or fibronectin content and cloning efficiency in agar was observed. Two hybrid lines, assayed for tumorigenicity in nude mice, did not produce tumors, even at inocula 20-fold greater than those at which the HeLa cells formed tumors.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019932986&partnerID=8YFLogxK
U2 - 10.1016/0277-5379(82)90194-8
DO - 10.1016/0277-5379(82)90194-8
M3 - Article
C2 - 6961038
AN - SCOPUS:0019932986
VL - 18
SP - 845
EP - 851
JO - European Journal of Cancer
JF - European Journal of Cancer
SN - 0959-8049
IS - 9
ER -