Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma

Giovanna Roncador, José Francisco García Verdes-Montenegro, Sara Tedoldi, Jennifer C. Paterson, Wolfram Klapper, Erica Ballabio, Lorena Maestre, Stefano Pileri, Martin Leo Hansmann, Miguel A. Piris, David Y. Mason, Teresa Marafioti

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Background and Objectives: In the present paper we report that SAP, an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers. Design and Methods: Expression of SAP and PD-1 was investigated by immunohistochemistry in paraffin-embedded tissue sections and in cell lines. Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells. Results: Screening on more than 500 lymphoma biopsies showed that 95% (40/42) of cases of AITL expressed at least one of these markers. SAP was also expressed on many cases (15/21) of acute T lymphoblastic leukemia, in keeping with its presence in cortical thymocytes. However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells. Interpretation and Conclusions: These two markers provide additional evidence that AITL arises from germinal center T cells. They may also prove of value in the diagnosis of this disease since a negative reaction was rarely observed in this disorder.

Original languageEnglish
Pages (from-to)1059-1066
Number of pages8
JournalHaematologica
Volume92
Issue number8
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Germinal Center
T-Cell Lymphoma
T-Lymphocytes
Lymphoid Tissue
Helper-Inducer T-Lymphocytes
Paraffin
Peripheral T-Cell Lymphoma
Cell Line
Antibody Specificity
Thymocytes
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Lymphoma
Western Blotting
Immunohistochemistry
Lymphocytes
Biopsy
Antibodies

Keywords

  • AITL
  • Germinal center T cells
  • Immunohistochemistry
  • Lymphoma
  • Western blotting

ASJC Scopus subject areas

  • Hematology

Cite this

Roncador, G., Verdes-Montenegro, J. F. G., Tedoldi, S., Paterson, J. C., Klapper, W., Ballabio, E., ... Marafioti, T. (2007). Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. Haematologica, 92(8), 1059-1066. https://doi.org/10.3324/haematol.10864

Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. / Roncador, Giovanna; Verdes-Montenegro, José Francisco García; Tedoldi, Sara; Paterson, Jennifer C.; Klapper, Wolfram; Ballabio, Erica; Maestre, Lorena; Pileri, Stefano; Hansmann, Martin Leo; Piris, Miguel A.; Mason, David Y.; Marafioti, Teresa.

In: Haematologica, Vol. 92, No. 8, 08.2007, p. 1059-1066.

Research output: Contribution to journalArticle

Roncador, G, Verdes-Montenegro, JFG, Tedoldi, S, Paterson, JC, Klapper, W, Ballabio, E, Maestre, L, Pileri, S, Hansmann, ML, Piris, MA, Mason, DY & Marafioti, T 2007, 'Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma', Haematologica, vol. 92, no. 8, pp. 1059-1066. https://doi.org/10.3324/haematol.10864
Roncador G, Verdes-Montenegro JFG, Tedoldi S, Paterson JC, Klapper W, Ballabio E et al. Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. Haematologica. 2007 Aug;92(8):1059-1066. https://doi.org/10.3324/haematol.10864
Roncador, Giovanna ; Verdes-Montenegro, José Francisco García ; Tedoldi, Sara ; Paterson, Jennifer C. ; Klapper, Wolfram ; Ballabio, Erica ; Maestre, Lorena ; Pileri, Stefano ; Hansmann, Martin Leo ; Piris, Miguel A. ; Mason, David Y. ; Marafioti, Teresa. / Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. In: Haematologica. 2007 ; Vol. 92, No. 8. pp. 1059-1066.
@article{41e166c1aa0542a3832a817fa596a947,
title = "Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma",
abstract = "Background and Objectives: In the present paper we report that SAP, an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers. Design and Methods: Expression of SAP and PD-1 was investigated by immunohistochemistry in paraffin-embedded tissue sections and in cell lines. Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells. Results: Screening on more than 500 lymphoma biopsies showed that 95{\%} (40/42) of cases of AITL expressed at least one of these markers. SAP was also expressed on many cases (15/21) of acute T lymphoblastic leukemia, in keeping with its presence in cortical thymocytes. However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells. Interpretation and Conclusions: These two markers provide additional evidence that AITL arises from germinal center T cells. They may also prove of value in the diagnosis of this disease since a negative reaction was rarely observed in this disorder.",
keywords = "AITL, Germinal center T cells, Immunohistochemistry, Lymphoma, Western blotting",
author = "Giovanna Roncador and Verdes-Montenegro, {Jos{\'e} Francisco Garc{\'i}a} and Sara Tedoldi and Paterson, {Jennifer C.} and Wolfram Klapper and Erica Ballabio and Lorena Maestre and Stefano Pileri and Hansmann, {Martin Leo} and Piris, {Miguel A.} and Mason, {David Y.} and Teresa Marafioti",
year = "2007",
month = "8",
doi = "10.3324/haematol.10864",
language = "English",
volume = "92",
pages = "1059--1066",
journal = "Haematologica",
issn = "0390-6078",
publisher = "NLM (Medline)",
number = "8",

}

TY - JOUR

T1 - Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma

AU - Roncador, Giovanna

AU - Verdes-Montenegro, José Francisco García

AU - Tedoldi, Sara

AU - Paterson, Jennifer C.

AU - Klapper, Wolfram

AU - Ballabio, Erica

AU - Maestre, Lorena

AU - Pileri, Stefano

AU - Hansmann, Martin Leo

AU - Piris, Miguel A.

AU - Mason, David Y.

AU - Marafioti, Teresa

PY - 2007/8

Y1 - 2007/8

N2 - Background and Objectives: In the present paper we report that SAP, an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers. Design and Methods: Expression of SAP and PD-1 was investigated by immunohistochemistry in paraffin-embedded tissue sections and in cell lines. Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells. Results: Screening on more than 500 lymphoma biopsies showed that 95% (40/42) of cases of AITL expressed at least one of these markers. SAP was also expressed on many cases (15/21) of acute T lymphoblastic leukemia, in keeping with its presence in cortical thymocytes. However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells. Interpretation and Conclusions: These two markers provide additional evidence that AITL arises from germinal center T cells. They may also prove of value in the diagnosis of this disease since a negative reaction was rarely observed in this disorder.

AB - Background and Objectives: In the present paper we report that SAP, an intracytoplasmic molecule that is involved in cell signaling, is an immunohistologic marker for germinal center T cells in paraffin-embedded tissue. We document its expression, and also that of PD-1 (another recently described marker of germinal center T cells to which a new antibody has been raised), in normal and neoplastic lymphoid tissue to evaluate the suggestion that helper T cells within the germinal centers of human lymphoid tissue are the cell of origin of angioimmunoblastic T-cell lymphoma (AITL), and to assess the diagnostic value of these two markers. Design and Methods: Expression of SAP and PD-1 was investigated by immunohistochemistry in paraffin-embedded tissue sections and in cell lines. Western blotting was performed on cell lines, and antibody specificity was confirmed by immunostaining of transfected cells. Results: Screening on more than 500 lymphoma biopsies showed that 95% (40/42) of cases of AITL expressed at least one of these markers. SAP was also expressed on many cases (15/21) of acute T lymphoblastic leukemia, in keeping with its presence in cortical thymocytes. However, PD-1 and SAP were also found in a minority of cases of peripheral T-cell lymphoma other than AITL, in contrast to a report that the former marker is specific for AITL. This observation raises the possibility that such non-angioimmunoblastic cases may be related to germinal center helper T cells. Interpretation and Conclusions: These two markers provide additional evidence that AITL arises from germinal center T cells. They may also prove of value in the diagnosis of this disease since a negative reaction was rarely observed in this disorder.

KW - AITL

KW - Germinal center T cells

KW - Immunohistochemistry

KW - Lymphoma

KW - Western blotting

UR - http://www.scopus.com/inward/record.url?scp=35748959075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748959075&partnerID=8YFLogxK

U2 - 10.3324/haematol.10864

DO - 10.3324/haematol.10864

M3 - Article

C2 - 17640856

AN - SCOPUS:35748959075

VL - 92

SP - 1059

EP - 1066

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 8

ER -