Expression of type X collagen is transiently stimulated in redifferentiating chondrocytes pretreated with retinoic acid

M. Sanchez, E. Gionti, G. Pontarelli, A. Arcella, F. De Lorenzo

Research output: Contribution to journalArticlepeer-review

Abstract

Growth of quail chondrocytes in the presence of retinoic acid (RA) results in the suppression of the differentiated phenotype. RA-treated chondrocytes recover their differentiated phenotype if they are cultured for an additional 15 days in the absence of RA. A few days after removal from RA, treated chondrocytes acquire the polygonal morphology characteristic of chondrocytes growing as attached cells; they also gradually resume collagen II expression and synthesize a significantly higher proportion of collagen X mRNA than do untreated control cultures. The levels of collagen X mRNA decrease during the second week of culture in the absence of RA. Finally, at the end of 15 days, the absolute levels of collagen II and collagen X mRNAs are very similar in control and recovering chondrocytes.

Original languageEnglish
Pages (from-to)183-187
Number of pages5
JournalBiochemical Journal
Volume276
Issue number1
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Biochemistry

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