Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

Fabio L M Ricciardolo, Federica Sabatini, Valentina Sorbello, Sabrina Benedetto, Ilaria Defilippi, Loredana Petecchia, Cesare Usai, Isabella Gnemmi, Bruno Balbi, Virginia De Rose, Nick H T Ten Hacken, Dirkje S. Postma, Wim Timens, Antonino Di Stefano

Research output: Contribution to journalArticle

Abstract

Background Vascular remodelling plays a central role in asthma and chronic obstructive pulmonary disease (COPD). Bradykinin (BK) is a vasoactive proinflammatory peptide mediating acute responses in asthma. We investigated the role of angiogenic factors in relation to BK receptors in asthma and COPD. Methods Bronchial biopsies from 33 patients with COPD, 24 old (≥50 years) patients with (≥50 years) asthma, 18 old control smokers, 11 old control nonsmokers, 15 young (≤40yrs) patients with (≤40 years) asthma and 10 young control non-smokers were immunostained for CD31, vascular endothelial growth factor-A (VEGF-A), angiogenin and BK receptors (B2R and B1R). Fibroblast and endothelial co-localisation of relevant molecules were performed by immunofluorescence. BK-induced VEGF-A and angiogenin release was studied (ELISA) in bronchial fibroblasts from subjects with asthma and COPD. Results In bronchial lamina propria of old patients with asthma, CD31 and VEGF-A+ cell numbers were higher than old control non-smokers ( p

Original languageEnglish
Pages (from-to)803-811
Number of pages9
JournalThorax
Volume68
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Bradykinin Receptors
Chronic Obstructive Pulmonary Disease
Asthma
Vascular Endothelial Growth Factor A
Bradykinin
Fibroblasts
Vascular Endothelial Growth Factor Receptor
Angiogenesis Inducing Agents
Vascular Remodeling
Fluorescent Antibody Technique
Mucous Membrane
Cell Count
Enzyme-Linked Immunosorbent Assay
Biopsy
Peptides

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD. / Ricciardolo, Fabio L M; Sabatini, Federica; Sorbello, Valentina; Benedetto, Sabrina; Defilippi, Ilaria; Petecchia, Loredana; Usai, Cesare; Gnemmi, Isabella; Balbi, Bruno; De Rose, Virginia; Ten Hacken, Nick H T; Postma, Dirkje S.; Timens, Wim; Di Stefano, Antonino.

In: Thorax, Vol. 68, No. 9, 09.2013, p. 803-811.

Research output: Contribution to journalArticle

Ricciardolo, FLM, Sabatini, F, Sorbello, V, Benedetto, S, Defilippi, I, Petecchia, L, Usai, C, Gnemmi, I, Balbi, B, De Rose, V, Ten Hacken, NHT, Postma, DS, Timens, W & Di Stefano, A 2013, 'Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD', Thorax, vol. 68, no. 9, pp. 803-811. https://doi.org/10.1136/thoraxjnl-2012-202741
Ricciardolo, Fabio L M ; Sabatini, Federica ; Sorbello, Valentina ; Benedetto, Sabrina ; Defilippi, Ilaria ; Petecchia, Loredana ; Usai, Cesare ; Gnemmi, Isabella ; Balbi, Bruno ; De Rose, Virginia ; Ten Hacken, Nick H T ; Postma, Dirkje S. ; Timens, Wim ; Di Stefano, Antonino. / Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD. In: Thorax. 2013 ; Vol. 68, No. 9. pp. 803-811.
@article{b5fea917c1e04c3fbc15474c33c4d089,
title = "Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD",
abstract = "Background Vascular remodelling plays a central role in asthma and chronic obstructive pulmonary disease (COPD). Bradykinin (BK) is a vasoactive proinflammatory peptide mediating acute responses in asthma. We investigated the role of angiogenic factors in relation to BK receptors in asthma and COPD. Methods Bronchial biopsies from 33 patients with COPD, 24 old (≥50 years) patients with (≥50 years) asthma, 18 old control smokers, 11 old control nonsmokers, 15 young (≤40yrs) patients with (≤40 years) asthma and 10 young control non-smokers were immunostained for CD31, vascular endothelial growth factor-A (VEGF-A), angiogenin and BK receptors (B2R and B1R). Fibroblast and endothelial co-localisation of relevant molecules were performed by immunofluorescence. BK-induced VEGF-A and angiogenin release was studied (ELISA) in bronchial fibroblasts from subjects with asthma and COPD. Results In bronchial lamina propria of old patients with asthma, CD31 and VEGF-A+ cell numbers were higher than old control non-smokers ( p",
author = "Ricciardolo, {Fabio L M} and Federica Sabatini and Valentina Sorbello and Sabrina Benedetto and Ilaria Defilippi and Loredana Petecchia and Cesare Usai and Isabella Gnemmi and Bruno Balbi and {De Rose}, Virginia and {Ten Hacken}, {Nick H T} and Postma, {Dirkje S.} and Wim Timens and {Di Stefano}, Antonino",
year = "2013",
month = "9",
doi = "10.1136/thoraxjnl-2012-202741",
language = "English",
volume = "68",
pages = "803--811",
journal = "Thorax",
issn = "0040-6376",
publisher = "BMJ Publishing Group",
number = "9",

}

TY - JOUR

T1 - Expression of vascular remodelling markers in relation to bradykinin receptors in asthma and COPD

AU - Ricciardolo, Fabio L M

AU - Sabatini, Federica

AU - Sorbello, Valentina

AU - Benedetto, Sabrina

AU - Defilippi, Ilaria

AU - Petecchia, Loredana

AU - Usai, Cesare

AU - Gnemmi, Isabella

AU - Balbi, Bruno

AU - De Rose, Virginia

AU - Ten Hacken, Nick H T

AU - Postma, Dirkje S.

AU - Timens, Wim

AU - Di Stefano, Antonino

PY - 2013/9

Y1 - 2013/9

N2 - Background Vascular remodelling plays a central role in asthma and chronic obstructive pulmonary disease (COPD). Bradykinin (BK) is a vasoactive proinflammatory peptide mediating acute responses in asthma. We investigated the role of angiogenic factors in relation to BK receptors in asthma and COPD. Methods Bronchial biopsies from 33 patients with COPD, 24 old (≥50 years) patients with (≥50 years) asthma, 18 old control smokers, 11 old control nonsmokers, 15 young (≤40yrs) patients with (≤40 years) asthma and 10 young control non-smokers were immunostained for CD31, vascular endothelial growth factor-A (VEGF-A), angiogenin and BK receptors (B2R and B1R). Fibroblast and endothelial co-localisation of relevant molecules were performed by immunofluorescence. BK-induced VEGF-A and angiogenin release was studied (ELISA) in bronchial fibroblasts from subjects with asthma and COPD. Results In bronchial lamina propria of old patients with asthma, CD31 and VEGF-A+ cell numbers were higher than old control non-smokers ( p

AB - Background Vascular remodelling plays a central role in asthma and chronic obstructive pulmonary disease (COPD). Bradykinin (BK) is a vasoactive proinflammatory peptide mediating acute responses in asthma. We investigated the role of angiogenic factors in relation to BK receptors in asthma and COPD. Methods Bronchial biopsies from 33 patients with COPD, 24 old (≥50 years) patients with (≥50 years) asthma, 18 old control smokers, 11 old control nonsmokers, 15 young (≤40yrs) patients with (≤40 years) asthma and 10 young control non-smokers were immunostained for CD31, vascular endothelial growth factor-A (VEGF-A), angiogenin and BK receptors (B2R and B1R). Fibroblast and endothelial co-localisation of relevant molecules were performed by immunofluorescence. BK-induced VEGF-A and angiogenin release was studied (ELISA) in bronchial fibroblasts from subjects with asthma and COPD. Results In bronchial lamina propria of old patients with asthma, CD31 and VEGF-A+ cell numbers were higher than old control non-smokers ( p

UR - http://www.scopus.com/inward/record.url?scp=84881548755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881548755&partnerID=8YFLogxK

U2 - 10.1136/thoraxjnl-2012-202741

DO - 10.1136/thoraxjnl-2012-202741

M3 - Article

C2 - 23739138

AN - SCOPUS:84881548755

VL - 68

SP - 803

EP - 811

JO - Thorax

JF - Thorax

SN - 0040-6376

IS - 9

ER -