Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: Colocalization with NEDD8

Salvatore Carrabino, Emanuela Carminati, Daniela Talarico, Ruggero Pardi, Elisabetta Bianchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The COP9 signalosome (CSN) is a conserved multiprotein complex, with an important developmental role in several organisms, ranging from plants to mammalians. The influence of the CSN on several signaling and developmental processes has been ascribed to its ability to regulate degradation of a number of signaling proteins by the ubiquitin-proteasome system. The CSN controls the function of the SCF ubiquitin-ligase complex through an enzymatic activity that removes the small ubiquitin-like molecule NEDD8 from the cullin component of the SCF and that requires subunit 5 of the CSN (JAB1/CSN5). Mutants of the CSN display early embryonic lethality, a feature that has hindered further characterization of the role of the CSN at later stages of mammalian development. Here we report the analysis of JAB1/CSN5 expression pattern in the mouse embryo. At early stages of development, JAB1/CSN5 transcripts were present with low expression levels in all tissues. Preferential expression in selected tissues was detected starting at E11.5, with higher levels in dorsal root ganglia; at later stages, prominent expression of JAB1/CSN5 transcripts was observed in cranial nerve, spinal and sympathetic ganglia, as well as in selected epithelia, such as the oral and the olfactory epithelium. In the adult brain, additional areas of JAB1/CSN5 expression were the hippocampus and the Purkinjie layer of the cerebellum. We also analyzed the temporal and spatial expression pattern of NEDD8, and found that it substantially overlapped JAB1/CSN5 expression at all stages analyzed, supporting the model of a functional interaction between the two proteins during developmental processes.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalGene Expression Patterns
Volume4
Issue number4
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Embryonic Development
Genes
Spinal Ganglia
Ubiquitin
SKP Cullin F-Box Protein Ligases
Cullin Proteins
Multiprotein Complexes
Olfactory Mucosa
Sympathetic Ganglia
Cranial Nerves
Proteasome Endopeptidase Complex
Cerebellum
COP9 signalosome complex
Hippocampus
Proteins
Embryonic Structures
Epithelium
Brain

Keywords

  • COP9 signalosome
  • Cranial nerve-associated ganglia
  • Embryonic development
  • Hippocampus
  • In situ hybridization
  • JAB1/CSN5
  • Mouse
  • Nasal epithelium
  • NEDD8
  • Purkinjie

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Expression pattern of the JAB1/CSN5 gene during murine embryogenesis : Colocalization with NEDD8. / Carrabino, Salvatore; Carminati, Emanuela; Talarico, Daniela; Pardi, Ruggero; Bianchi, Elisabetta.

In: Gene Expression Patterns, Vol. 4, No. 4, 07.2004, p. 423-431.

Research output: Contribution to journalArticle

Carrabino, Salvatore ; Carminati, Emanuela ; Talarico, Daniela ; Pardi, Ruggero ; Bianchi, Elisabetta. / Expression pattern of the JAB1/CSN5 gene during murine embryogenesis : Colocalization with NEDD8. In: Gene Expression Patterns. 2004 ; Vol. 4, No. 4. pp. 423-431.
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