Expression pattern of the RARα-PML fusion gene in acute promyelocytic leukemia

Myriam Alcalay, Daniela Zangrilli, Marta Fagioli, Pier Paolo Pandolfi, Amedea Mencarelli, Francesco Lo Coco, Andrea Biondi, Fausto Grignani, Pier Giuseppe Pelicci

Research output: Contribution to journalArticle

Abstract

Two chimeric genes, PML-RARα and RARα-PML, are formed as a consequence of the acute promyelocytic leukemia (APL)-specific reciprocal translocation of chromosomes 15 and 17 [t(15;17)]. PML-RARα is expressed as a fusion protein. We investigated the organization and expression pattern of the RARα-PML gene in a series of APL patients representative of the molecular heterogeneity of the t(15;17) and found (i) two types of RARα-PML mRNA junctions (RARα exon 2/PML exon 4 or RARα exon 2/PML exon 7) that maintain the RARα and PML longest open reading frames aligned and are the result of chromosome 15 breaking at two different sites; and (ii) 10 different RARα-PML fusion transcripts that differ for the assembly of their PML coding exons. A RARα-PML transcript was present in most, but not all, APL patients.

Original languageEnglish
Pages (from-to)4840-4844
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number11
Publication statusPublished - 1992

Keywords

  • Leukemogenesis
  • Reciprocal translocation
  • Transcription factors

ASJC Scopus subject areas

  • Genetics
  • General

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