Two chimeric genes, PML-RARα and RARα-PML, are formed as a consequence of the acute promyelocytic leukemia (APL)-specific reciprocal translocation of chromosomes 15 and 17 [t(15;17)]. PML-RARα is expressed as a fusion protein. We investigated the organization and expression pattern of the RARα-PML gene in a series of APL patients representative of the molecular heterogeneity of the t(15;17) and found (i) two types of RARα-PML mRNA junctions (RARα exon 2/PML exon 4 or RARα exon 2/PML exon 7) that maintain the RARα and PML longest open reading frames aligned and are the result of chromosome 15 breaking at two different sites; and (ii) 10 different RARα-PML fusion transcripts that differ for the assembly of their PML coding exons. A RARα-PML transcript was present in most, but not all, APL patients.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Publication status||Published - 1992|
- Reciprocal translocation
- Transcription factors
ASJC Scopus subject areas