Expression profiling of microRNA using oligo DNA arrays

Chang Gong Liu, Riccardo Spizzo, George Adrian Calin, Carlo Maria Croce

Research output: Contribution to journalArticlepeer-review

Abstract

After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of non-coding RNA (ncRNA) in different human diseases. The microRNAs (miRNAs) are one of the groups of ncRNA. They are small RNA fragments, 19-25 nucleotides long, with a main regulatory function on both protein coding genes and non-coding RNAs. The application of microarray platforms applied to miRNA profiling determined their deregulation in virtually all human diseases that have been studied. We previously developed a custom miRNA microarray platform, and here we describe the protocol we used to work with it including the oligo design strategy, the microarray printing protocol, the target-probe hybridization and the signal detection.

Original languageEnglish
Pages (from-to)22-30
Number of pages9
JournalMethods
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Cancer
  • Microarray
  • microRNA
  • Non-coding RNA
  • Other diseases
  • Tumor profilling

ASJC Scopus subject areas

  • Molecular Biology

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