Expression, purification, and functional characterization of a Kunitz-type module from chicken type VI collagen

A. Bearz, G. Tolazzi, A. Leonardi, C. Pucillo, G. Tell, A. Colombatti, S. Formisano

Research output: Contribution to journalArticle

Abstract

The primary amino acid sequence of the carboxyl-terminal portion of the α3 chain of chicken type VI collagen (K-VI) presents a 58-residue motif with a high degree of homology with members of the Kunitz serine-proteinase inhibitors family. This module was cloned, expressed in E. coli, purified and compared to the bovine pancreatic trypsin inhibitor (BPTI) in an inhibition profile assay of two serine proteases, trypsin and plasmin. We found that recombinant K-VI is not endowed with inhibitory activity but it slightly activates both plasmin and trypsin, differently from other members of the family. Moreover, the ability to inhibit the serine protease activity is also lacking in the intact type VI collagen molecule.

Original languageEnglish
Pages (from-to)1050-1055
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume215
Issue number3
DOIs
Publication statusPublished - 1995

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Collagen Type VI
Fibrinolysin
Serine Proteases
Trypsin
Purification
Chickens
Serine Proteinase Inhibitors
Aprotinin
Aptitude
Escherichia coli
Amino Acid Sequence
Assays
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Expression, purification, and functional characterization of a Kunitz-type module from chicken type VI collagen. / Bearz, A.; Tolazzi, G.; Leonardi, A.; Pucillo, C.; Tell, G.; Colombatti, A.; Formisano, S.

In: Biochemical and Biophysical Research Communications, Vol. 215, No. 3, 1995, p. 1050-1055.

Research output: Contribution to journalArticle

Bearz, A. ; Tolazzi, G. ; Leonardi, A. ; Pucillo, C. ; Tell, G. ; Colombatti, A. ; Formisano, S. / Expression, purification, and functional characterization of a Kunitz-type module from chicken type VI collagen. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 215, No. 3. pp. 1050-1055.
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