Human hexokinase type I, one of the four isozymes consisting of a single polypeptide chain of about 100 kDa, has been cloned in the pET expression plasmid in a truncated form lacking a segment of 11 apolar amino acids at the N-terminus. The protein has been overexpressed in E.coli and purified to homogeneity. Truncated hexokinase I has been crystallized in the presence of glucose 6-phosphate and of the ATP analogue AMP-PNP. The crystals belong to the monoclinic space group P21, with unit cell constants: a = 84.2 Å, b = 177.7 Å, c = 88.2 Å, β = 90.8° and diffract to 3.0 A resolution.
|Number of pages||6|
|Journal||Protein and Peptide Letters|
|Publication status||Published - 1998|
ASJC Scopus subject areas
- Molecular Biology