Expression, purification, crystallization and preliminary X-ray analysis of the augmenter of liver regeneration

Chia Kuei Wu, Tamara A. Dailey, Harry A. Dailey, Antonio Francavilla, Thomas E. Starzl, Bi Cheng Wang, John P. Rose

Research output: Contribution to journalArticlepeer-review

Abstract

A novel growth factor termed augmenter of liver regeneration (ALR) containing an N-terminal histidine tag has been expressed, purified and crystallized. The crystals diffract to beyond 2 Å, belong to space group I41 with a = 99.8Å and c = 113.4̊, and have four molecules per asymmetric unit. Cadmium proved essential to the crystallization of the protein and the positions of four cadmium atoms have been determined by Bijvoet difference Patterson analysis. A possible role for cadmium stabilization of the his-tagged protein lattice is discussed.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalProtein and Peptide Letters
Volume7
Issue number1
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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