Expression, regulation, and tissue distribution of the Ch21 protein during chicken embryogenesis

B. Dozin, F. Descalzi, L. Briata, M. Hayashi, C. Gentili, K. Hayashi, R. Quarto, R. Cancedda

Research output: Contribution to journalArticle

Abstract

The Ch21 protein is one of the marker proteins whose synthesis and secretion by differentiating tibia chondrocytes is progressively increased during chicken embryogenesis (Descalzi-Cancedda, F., Manduca, P., Tacchetti, C., Fossa, P., Quarto, R. and Cancedda, R. (1988) J. Cell Biol. 107, 2455- 2463). We report the corresponding full-length cDNA sequence and the complete primary structure of the protein, which contains a 20-residue signal peptide. The synthesis of the protein is directed by a 1450-base mRNA characterized by an unusually long 5'-untranslated leader sequence and is regulated only at the transcriptional level as shown by run-off transcription assays. During differentiation, maximal expression of the protein characterizes stage II hypertrophic chondrocytes. In situ hybridization on embryo sections reveals that the protein is expressed by several structures derived from the chondrogenic lineage and that, in addition, it is a major translational product in granulocytes. High cell density largely influences the expression of the Ch21 protein in chondrocyte cultures. When embryonic avian cells of different origin are grown to confluency, the expression of the Ch21 protein is observed in only some of the cell lines. Thus quiescence per se is not the primary factor determining the expression of the protein.

Original languageEnglish
Pages (from-to)2979-2985
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number5
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Biochemistry

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    Dozin, B., Descalzi, F., Briata, L., Hayashi, M., Gentili, C., Hayashi, K., Quarto, R., & Cancedda, R. (1992). Expression, regulation, and tissue distribution of the Ch21 protein during chicken embryogenesis. Journal of Biological Chemistry, 267(5), 2979-2985.