Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1

Tiziana Piepoli, Cemile Jakupoglu, Wenli Gu, Elena Lualdi, Blanca Suarez-Merino, Pietro L. Poliani, Maria Grazia Cattaneo, Barbara Ortino, Dorota Goplen, Jian Wang, Rosa Mola, Francesca Inverardi, Carolina Frassoni, Rolf Bjerkvig, Ortrud Steinlein, Lucia M. Vicentini, Oliver Brüstle, Gaetano Finocchiaro

Research output: Contribution to journalArticle

Abstract

Disruptions of LGI1 in glioblastoma (GBM) cell lines and LGI1 mutations in families with autosomal dominant epilepsy imply a role for LGI1 in glial cells as well as in neurons. Although we and others could not find LGI1 mutations in malignant gliomas, our initial studies appeared to support the idea that LGI1 is poorly expressed or absent in these tumors. Microarray data suggested that LGI1 could be involved in the control of matrix metalloproteinases, and we found that tumors derived from U87 glioblastoma cells overexpressing LGI1 were less aggressive than U87 control tumors. To our surprise, we observed that LGI1 expression after differentiation of murine neural stem cells was robust in neurons but negligible in glial cells, in agreement with immunohistochemistry studies on rodent brain. This observation could suggest that the variable levels of LGI1 expression in gliomas reflect the presence of neurons entrapped within the tumor. To test this hypothesis, we investigated LGI1 expression in parallel with expression of the neuronal marker NEF3 by real-time PCR on 30 malignant gliomas. Results showed a strong, positive correlation between the expression levels of these two genes (P <0.0001). Thus, our data confirm that LGI1 is involved in cell-matrix interactions but suggest that its expression is not relevant in glial cells, implying that its role as a tumor suppressor in gliomas should be reconsidered.

Original languageEnglish
Pages (from-to)96-108
Number of pages13
JournalNeuro-Oncology
Volume8
Issue number2
DOIs
Publication statusPublished - Apr 2006

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Glioma
Neuroglia
Neoplasms
Glioblastoma
Neurons
Mutation
Neural Stem Cells
Matrix Metalloproteinases
Cell Communication
Real-Time Polymerase Chain Reaction
Rodentia
Epilepsy
Immunohistochemistry
Cell Line
Brain
Genes

Keywords

  • Epilepsy
  • Glioma
  • Invasion
  • Metalloproteinase
  • Migration
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1. / Piepoli, Tiziana; Jakupoglu, Cemile; Gu, Wenli; Lualdi, Elena; Suarez-Merino, Blanca; Poliani, Pietro L.; Cattaneo, Maria Grazia; Ortino, Barbara; Goplen, Dorota; Wang, Jian; Mola, Rosa; Inverardi, Francesca; Frassoni, Carolina; Bjerkvig, Rolf; Steinlein, Ortrud; Vicentini, Lucia M.; Brüstle, Oliver; Finocchiaro, Gaetano.

In: Neuro-Oncology, Vol. 8, No. 2, 04.2006, p. 96-108.

Research output: Contribution to journalArticle

Piepoli, T, Jakupoglu, C, Gu, W, Lualdi, E, Suarez-Merino, B, Poliani, PL, Cattaneo, MG, Ortino, B, Goplen, D, Wang, J, Mola, R, Inverardi, F, Frassoni, C, Bjerkvig, R, Steinlein, O, Vicentini, LM, Brüstle, O & Finocchiaro, G 2006, 'Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1', Neuro-Oncology, vol. 8, no. 2, pp. 96-108. https://doi.org/10.1215/15228517-2005-006
Piepoli T, Jakupoglu C, Gu W, Lualdi E, Suarez-Merino B, Poliani PL et al. Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1. Neuro-Oncology. 2006 Apr;8(2):96-108. https://doi.org/10.1215/15228517-2005-006
Piepoli, Tiziana ; Jakupoglu, Cemile ; Gu, Wenli ; Lualdi, Elena ; Suarez-Merino, Blanca ; Poliani, Pietro L. ; Cattaneo, Maria Grazia ; Ortino, Barbara ; Goplen, Dorota ; Wang, Jian ; Mola, Rosa ; Inverardi, Francesca ; Frassoni, Carolina ; Bjerkvig, Rolf ; Steinlein, Ortrud ; Vicentini, Lucia M. ; Brüstle, Oliver ; Finocchiaro, Gaetano. / Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1. In: Neuro-Oncology. 2006 ; Vol. 8, No. 2. pp. 96-108.
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AU - Suarez-Merino, Blanca

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AU - Cattaneo, Maria Grazia

AU - Ortino, Barbara

AU - Goplen, Dorota

AU - Wang, Jian

AU - Mola, Rosa

AU - Inverardi, Francesca

AU - Frassoni, Carolina

AU - Bjerkvig, Rolf

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