Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

Bradley R. Groveman; Christina D. Orrù; Andrew G. Hughson; Matilde Bongianni; Michele Fiorini; Daniele Imperiale; Anna Ladogana; Maurizio Pocchiari; Gianluigi Zanusso; Byron Caughey

doi:10.1002/acn3.378

Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

Bradley R. Groveman, Christina D. Orrù, Andrew G. Hughson, Matilde Bongianni, Michele Fiorini, Daniele Imperiale, Anna Ladogana, Maurizio Pocchiari, Gianluigi Zanusso, Byron Caughey

Istituto Superiore di Sanita'

Research output: Contribution to journal › Article › peer-review

Abstract

Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.

Original language	English
Pages (from-to)	139-144
Number of pages	6
Journal	Annals of Clinical and Translational Neurology
Volume	4
Issue number	2
DOIs	https://doi.org/10.1002/acn3.378
Publication status	Published - Feb 1 2017

Keywords

Creutzfeld-Jakob disease
Diagnostic test

ASJC Scopus subject areas

Neuroscience(all)
Clinical Neurology

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10.1002/acn3.378

Cite this

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abstract = "Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.",

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T1 - Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

AU - Groveman, Bradley R.

AU - Orrù, Christina D.

AU - Hughson, Andrew G.

AU - Bongianni, Matilde

AU - Fiorini, Michele

AU - Imperiale, Daniele

AU - Ladogana, Anna

AU - Pocchiari, Maurizio

AU - Zanusso, Gianluigi

AU - Caughey, Byron

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AB - Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.

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Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

Abstract

Keywords

ASJC Scopus subject areas

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