Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

Bradley R. Groveman, Christina D. Orrù, Andrew G. Hughson, Matilde Bongianni, Michele Fiorini, Daniele Imperiale, Anna Ladogana, Maurizio Pocchiari, Gianluigi Zanusso, Byron Caughey

Research output: Contribution to journalArticle

Abstract

Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalAnnals of Clinical and Translational Neurology
Volume4
Issue number2
DOIs
Publication statusPublished - Feb 1 2017

Keywords

  • Creutzfeld-Jakob disease
  • Diagnostic test

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

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