Abstract
Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
| Original language | English |
|---|---|
| Pages (from-to) | 139-144 |
| Number of pages | 6 |
| Journal | Annals of Clinical and Translational Neurology |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2017 |
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Keywords
- Creutzfeld-Jakob disease
- Diagnostic test
ASJC Scopus subject areas
- Neuroscience(all)
- Clinical Neurology
Cite this
Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis. / Groveman, Bradley R.; Orrù, Christina D.; Hughson, Andrew G.; Bongianni, Matilde; Fiorini, Michele; Imperiale, Daniele; Ladogana, Anna; Pocchiari, Maurizio; Zanusso, Gianluigi; Caughey, Byron.
In: Annals of Clinical and Translational Neurology, Vol. 4, No. 2, 01.02.2017, p. 139-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis
AU - Groveman, Bradley R.
AU - Orrù, Christina D.
AU - Hughson, Andrew G.
AU - Bongianni, Matilde
AU - Fiorini, Michele
AU - Imperiale, Daniele
AU - Ladogana, Anna
AU - Pocchiari, Maurizio
AU - Zanusso, Gianluigi
AU - Caughey, Byron
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
AB - Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
KW - Creutzfeld-Jakob disease
KW - Diagnostic test
UR - http://www.scopus.com/inward/record.url?scp=85028061836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028061836&partnerID=8YFLogxK
U2 - 10.1002/acn3.378
DO - 10.1002/acn3.378
M3 - Article
AN - SCOPUS:85028061836
VL - 4
SP - 139
EP - 144
JO - Annals of Clinical and Translational Neurology
JF - Annals of Clinical and Translational Neurology
SN - 2328-9503
IS - 2
ER -