Abstract
Fifty faecal samples from healthy adults were grown on MacConkey agar and three pink colonies were subcultured, identified to species level and their antimicrobial susceptibility determined. Forty-seven samples yielded 141 isolates of Escherichia coli that were susceptible to most antimicrobials. Resistance was noted for ampicillin (30.5%), chloramphenicol (12.1%), tetracycline (23.4%), trimethoprim (24.8%) and co-trimoxazole (22.7%). A direct faecal plating method was used for extended resistance screening with E. coli as the indicator organism. Zone breakpoints were determined using normalised resistance interpretation and gave similar susceptibility results. Eighty-eight isolates of E. coli from within the zones of inhibition revealed four times more antimicrobial resistance. Extended antimicrobial resistance screening both provides the susceptibility profile of the dominant E. coli isolate and detects greater resistance in rare isolates.
| Original language | English |
|---|---|
| Pages (from-to) | 473-478 |
| Number of pages | 6 |
| Journal | International Journal of Antimicrobial Agents |
| Volume | 26 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2005 |
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Keywords
- Antimicrobial resistance surveillance
- Disk diffusion test
- Escherichia coli
- Interpretive zone breakpoints
- Normalised resistance interpretation
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Microbiology
- Parasitology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones. / Kronvall, Göran; Larsson, Mattias; Borén, Charlotte; Kahlmeter, Gunnar; Bartoloni, Alessandro; Rossolini, Gian Maria; Grape, Malin; Kristiansson, Charlotte; Karlsson, Inga.
In: International Journal of Antimicrobial Agents, Vol. 26, No. 6, 12.2005, p. 473-478.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones
AU - Kronvall, Göran
AU - Larsson, Mattias
AU - Borén, Charlotte
AU - Kahlmeter, Gunnar
AU - Bartoloni, Alessandro
AU - Rossolini, Gian Maria
AU - Grape, Malin
AU - Kristiansson, Charlotte
AU - Karlsson, Inga
PY - 2005/12
Y1 - 2005/12
N2 - Fifty faecal samples from healthy adults were grown on MacConkey agar and three pink colonies were subcultured, identified to species level and their antimicrobial susceptibility determined. Forty-seven samples yielded 141 isolates of Escherichia coli that were susceptible to most antimicrobials. Resistance was noted for ampicillin (30.5%), chloramphenicol (12.1%), tetracycline (23.4%), trimethoprim (24.8%) and co-trimoxazole (22.7%). A direct faecal plating method was used for extended resistance screening with E. coli as the indicator organism. Zone breakpoints were determined using normalised resistance interpretation and gave similar susceptibility results. Eighty-eight isolates of E. coli from within the zones of inhibition revealed four times more antimicrobial resistance. Extended antimicrobial resistance screening both provides the susceptibility profile of the dominant E. coli isolate and detects greater resistance in rare isolates.
AB - Fifty faecal samples from healthy adults were grown on MacConkey agar and three pink colonies were subcultured, identified to species level and their antimicrobial susceptibility determined. Forty-seven samples yielded 141 isolates of Escherichia coli that were susceptible to most antimicrobials. Resistance was noted for ampicillin (30.5%), chloramphenicol (12.1%), tetracycline (23.4%), trimethoprim (24.8%) and co-trimoxazole (22.7%). A direct faecal plating method was used for extended resistance screening with E. coli as the indicator organism. Zone breakpoints were determined using normalised resistance interpretation and gave similar susceptibility results. Eighty-eight isolates of E. coli from within the zones of inhibition revealed four times more antimicrobial resistance. Extended antimicrobial resistance screening both provides the susceptibility profile of the dominant E. coli isolate and detects greater resistance in rare isolates.
KW - Antimicrobial resistance surveillance
KW - Disk diffusion test
KW - Escherichia coli
KW - Interpretive zone breakpoints
KW - Normalised resistance interpretation
UR - http://www.scopus.com/inward/record.url?scp=27744499795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27744499795&partnerID=8YFLogxK
U2 - 10.1016/j.ijantimicag.2005.09.005
DO - 10.1016/j.ijantimicag.2005.09.005
M3 - Article
C2 - 16280241
AN - SCOPUS:27744499795
VL - 26
SP - 473
EP - 478
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
SN - 0924-8579
IS - 6
ER -