Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones

Göran Kronvall, Mattias Larsson, Charlotte Borén, Gunnar Kahlmeter, Alessandro Bartoloni, Gian Maria Rossolini, Malin Grape, Charlotte Kristiansson, Inga Karlsson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fifty faecal samples from healthy adults were grown on MacConkey agar and three pink colonies were subcultured, identified to species level and their antimicrobial susceptibility determined. Forty-seven samples yielded 141 isolates of Escherichia coli that were susceptible to most antimicrobials. Resistance was noted for ampicillin (30.5%), chloramphenicol (12.1%), tetracycline (23.4%), trimethoprim (24.8%) and co-trimoxazole (22.7%). A direct faecal plating method was used for extended resistance screening with E. coli as the indicator organism. Zone breakpoints were determined using normalised resistance interpretation and gave similar susceptibility results. Eighty-eight isolates of E. coli from within the zones of inhibition revealed four times more antimicrobial resistance. Extended antimicrobial resistance screening both provides the susceptibility profile of the dominant E. coli isolate and detects greater resistance in rare isolates.

Original languageEnglish
Pages (from-to)473-478
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume26
Issue number6
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Clone Cells
Escherichia coli
Trimethoprim
Sulfamethoxazole Drug Combination Trimethoprim
Chloramphenicol
Ampicillin
Tetracycline
Agar

Keywords

  • Antimicrobial resistance surveillance
  • Disk diffusion test
  • Escherichia coli
  • Interpretive zone breakpoints
  • Normalised resistance interpretation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones. / Kronvall, Göran; Larsson, Mattias; Borén, Charlotte; Kahlmeter, Gunnar; Bartoloni, Alessandro; Rossolini, Gian Maria; Grape, Malin; Kristiansson, Charlotte; Karlsson, Inga.

In: International Journal of Antimicrobial Agents, Vol. 26, No. 6, 12.2005, p. 473-478.

Research output: Contribution to journalArticle

Kronvall, G, Larsson, M, Borén, C, Kahlmeter, G, Bartoloni, A, Rossolini, GM, Grape, M, Kristiansson, C & Karlsson, I 2005, 'Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones', International Journal of Antimicrobial Agents, vol. 26, no. 6, pp. 473-478. https://doi.org/10.1016/j.ijantimicag.2005.09.005
Kronvall, Göran ; Larsson, Mattias ; Borén, Charlotte ; Kahlmeter, Gunnar ; Bartoloni, Alessandro ; Rossolini, Gian Maria ; Grape, Malin ; Kristiansson, Charlotte ; Karlsson, Inga. / Extended antimicrobial resistance screening of the dominant faecal Escherichia coli and of rare resistant clones. In: International Journal of Antimicrobial Agents. 2005 ; Vol. 26, No. 6. pp. 473-478.
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