Extended-spectrum β-lactamases from Klebsiella pneumoniae strains isolated at an Italian hospital

L. Pagani, P. Ronza, E. Giacobone, E. Romero

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Eighteen strains of Klebsiella pneumoniae recently isolated from hospitalized patients were resistant or moderately resistant to oxyimino-cephalosporins (ceftazidime and/or cefotaxime), aztreonam, cefoxitin and all but one were susceptible to imipenem. Analysis of enzymes produced by these clinical isolates revealed a wide pattern of extended-spectrum β-lactamases. All isolates produced one or more β-lactamases that were characterized preliminarily by their isoelectric point. Strains isolated early were from patients in the Intensive Care Unit and produced an ESβ-lactamase with an apparent pI of 7.6, whereas the later isolates were from surgical and medical wards of the same hospital and produced ESβ-lactamases with apparent pI of 8.2 and 8.4, repectively. This suggests the emergence of SHV-5 and MIR-1 β-lactamases in our hospital. Agarose gel electrophoresis of plasmid DNA revealed the presence of a similar plasmid of approximate size 60 Kb in all isolates.

Original languageEnglish
Pages (from-to)533-540
Number of pages8
JournalEuropean Journal of Epidemiology
Volume10
Issue number5
DOIs
Publication statusPublished - Oct 1994

Fingerprint

Klebsiella pneumoniae
Plasmids
Aztreonam
Cefoxitin
Ceftazidime
Cefotaxime
Agar Gel Electrophoresis
Imipenem
Isoelectric Point
Cephalosporins
Intensive Care Units
DNA
Enzymes

Keywords

  • Aztreonam resistance
  • Cefoxitin resistance
  • Extended-spectrum β-lactamases
  • Klebsiella pneumoniae
  • Oxyimino-cephalosporins resistance

ASJC Scopus subject areas

  • Epidemiology

Cite this

Extended-spectrum β-lactamases from Klebsiella pneumoniae strains isolated at an Italian hospital. / Pagani, L.; Ronza, P.; Giacobone, E.; Romero, E.

In: European Journal of Epidemiology, Vol. 10, No. 5, 10.1994, p. 533-540.

Research output: Contribution to journalArticle

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