Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores

Enhanced transfer in the presence of multiple acceptors

Badri P. Maliwal, Sangram Raut, Rafal Fudala, Sabato D'Auria, Vincenzo M. Marzullo, Alberto Luini, Ignacy Gryczynski, Zygmunt Gryczynski

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using commercially available organic fluorophores, the current applications of Förster (fluorescence) resonance energy transfer (FRET) are limited to about 80 Å. However, many essential activities in cells are spatially and/or temporally dependent on the assembly/disassembly of transient complexes consisting of large-size macromolecules that are frequently separated by distances greater than 100 Å. Expanding the accessible range for FRET to 150 Å would open up many cellular interactions to fluorescence and fluorescence-lifetime imaging. Here, we demonstrate that the use of multiple randomly distributed acceptors on proteins/antibodies, rather than the use of a single localized acceptor, makes it possible to significantly enhance FRET and detect interactions between the donor fluorophore and the acceptor-labeled protein at distances greater than 100 Å. A simple theoretical model for spherical bodies that have been randomly labeled with acceptors has been developed. To test the theoretical predictions of this system, we carried out FRET studies using a 30-mer oligonucleotide-avidin system that was labeled with the acceptors DyLight649 or Dylight750. The opposite 5'-end of the oligonucleotide was labeled with the Alexa568 donor. We observed significantly enhanced energy transfer due to presence of multiple acceptors on the avidin protein. The results and simulation indicate that use of a nanosized body that has been randomly labeled with multiple acceptors allows FRET measurements to be extended to over 150 Å when using commercially available probes and established protein-labeling protocols.

Original languageEnglish
Article number011006
JournalJournal of Biomedical Optics
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Fluorophores
Energy transfer
energy transfer
resonance fluorescence
Proteins
Avidin
Oligonucleotides
proteins
oligonucleotides
Macromolecules
Antibodies
Labeling
antibodies
Imaging techniques
macromolecules
marking
Fluorescence Resonance Energy Transfer
assembly
interactions
life (durability)

Keywords

  • DNA
  • Enhanced transfer efficiency
  • FRET
  • Interactions at distances greater than 100 Å
  • Multiple acceptors

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores : Enhanced transfer in the presence of multiple acceptors. / Maliwal, Badri P.; Raut, Sangram; Fudala, Rafal; D'Auria, Sabato; Marzullo, Vincenzo M.; Luini, Alberto; Gryczynski, Ignacy; Gryczynski, Zygmunt.

In: Journal of Biomedical Optics, Vol. 17, No. 1, 011006, 01.2012.

Research output: Contribution to journalArticle

Maliwal, Badri P. ; Raut, Sangram ; Fudala, Rafal ; D'Auria, Sabato ; Marzullo, Vincenzo M. ; Luini, Alberto ; Gryczynski, Ignacy ; Gryczynski, Zygmunt. / Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores : Enhanced transfer in the presence of multiple acceptors. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 1.
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